Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 10, Problem 12TF
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PCR is a useful tool for early diagnosis of an infectious disease.Elaborate.
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Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
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- describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.arrow_forwardWhen using a probe to detect an organism, why is it necessary to have some idea as to the organism’s identity?arrow_forwardWhat method is used to transfer plasmid DNA into bacteria? Group of answer choices heat shock treatment biolistics electroporation microinjectionarrow_forward
- Why are the following reagents used? Neutralizing solution (Plasmid isolation) Isopropanol (Plasmid isolation) RNase (isolation of genomic DNA)arrow_forwardSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forwardA technique used to determine the tertiary structure of a protein is— high-performance liquid chromatography. high-resolution electrophoresis. X-ray crystallography. ELISA. PCR.arrow_forward
- DNA can be separated based on fragment size using ________. a. restriction enzymes b. gel electrophoresis c. PCRarrow_forwardPCR can be used to identify an unknown bacterium because ?arrow_forwardGive advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.arrow_forward
- Which technique used to manipulate genetic material results in a significant increase in DNA or RNA fragments? Group of answer choices heat shock treatment electroporation gel electrophoresis polymerase chain reactionarrow_forwardEnzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonucleasearrow_forwardWhat is PCR? Why does Taq polymerase work better than a typical DNA Polymerase isolated from E. coli for PCR? The optimal growth temperature for E Coli is 37 °C.arrow_forward
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