Genetics: From Genes To Genomes (6th International Edition)
6th Edition
ISBN: 9781260041217
Author: Leland Hartwell Dr., ? Michael L. Goldberg Professor Dr., ? Janice Fischer, ? Leroy Hood Dr.
Publisher: Mcgraw-Hill
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Textbook Question
Chapter 10, Problem 7P
Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas geneticists studying bacteria almost never do? Why might bacterial geneticists have difficulties constructing cDNA libraries even if they wanted to?
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What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned
a heat shock gene from the bacteria for further analysis. After cloning, he isolated the plasmid
carrying his gene of interest for sequencing. Jackson finally received the nucleotide sequence
of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his
gene of interest using the bioinformatics databases available.
What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria? How will you identify clones of interest?
Chapter 10 Solutions
Genetics: From Genes To Genomes (6th International Edition)
Ch. 10 - Prob. 1PCh. 10 - List three independent techniques you could use to...Ch. 10 - Figure 10.2a has numbers indicating the...Ch. 10 - Which of the enzymes from the following list would...Ch. 10 - Prob. 5PCh. 10 - a. What sequence information about a gene is...Ch. 10 - Why do geneticists studying eukaryotic organisms...Ch. 10 - Consider three different kinds of human libraries:...Ch. 10 - The human genome has been sequenced, but we still...Ch. 10 - This problem investigates issues encountered in...
Ch. 10 - For the sake of simplicity, Fig. 10.4 omitted one...Ch. 10 - Give two different reasons for the much higher...Ch. 10 - Using a cDNA library, you isolated two different...Ch. 10 - The figure that follows shows part of a modified...Ch. 10 - In Problem 14, cDNAs F and G could not be found in...Ch. 10 - Fig. 10.10 presents a model for exon shuffling in...Ch. 10 - An interesting phenomenon found in vertebrate DNA...Ch. 10 - a. If you found a zinc-finger domain which...Ch. 10 - Prob. 19PCh. 10 - In the human immune system, so-called B cells can...Ch. 10 - Chimpanzees have a set of hemoglobin genes very...Ch. 10 - Complete genome sequences indicate that the human...Ch. 10 - On your computers browser, view the page accessed...Ch. 10 - Prob. 24PCh. 10 - Prob. 25PCh. 10 - Certain individuals with mild forms of...Ch. 10 - The 1 and 2 genes in humans are identical in their...Ch. 10 - Prob. 28P
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- What is an advantage of making a cDNA library rather than a genomic library?arrow_forwardWhy is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?arrow_forwardIhsan is a biologist working with the genetics of a psychrophilic bacterium. He cloned an antifreeze gene from the bacteria for further analysis. After cloning, he isolated the plasmid carrying his gene of interest for sequencing. Ihsan finally received the nucleotide sequence of his gene. Explain in detail how he could verify whether the nucleotide sequence matches his gene of interest using the bioinformatics databases available.arrow_forward
- I recently isolated the human enzyme called fucosidase and prepared anantibody to it. Now I want to isolate a cDNA clone coding for this enzyme from a human cDNAlibrary. A friend of mine in the lab next door has informed me that he had recently isolated acDNA coding for dog fucosidase that I can use if I desire. In addition, I just read an article whichreported the sequence of the first 20 amino acids of human fucosidase. Which of the probeslisted below do you think I could use to screen my library to identify the cDNA clone containinghuman fucosidase?arrow_forwardDescribe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?arrow_forwardApart from genome size, what factors make completeassembly of a eukaryotic genome more difficult than assemblyof a genome from a species of Bacteria or Archaea?arrow_forward
- Although a number of different animals have been successfully cloned, the process of creating cloned animals is very inefficient. Typically, only about 0.1% to 3% of attempts result in a live-born animal. What might be some of the reasons for this low rate of success?arrow_forwardIn 1995, the first free-living organism to have its genome completely sequenced was Haemophilus influenzae, a bacteria. In the following year, the baker’s yeast Saccharomyces cerevisiae was the first eukaryote genome sequence to be fully sequenced. The complete sequencing of the human genome and related organisms represent one of the greatest scientific achievements in the history of mankind.Elaborate on the importance of genome studies in general.arrow_forwardThe genome of a typical bacterium contains about 5 x 106 base pairs, and can be replicated in about 30 minutes. The human genome is 600 times larger (3 × 10° base pairs), and at the rate of a bacterium, it would require 300 hours (~12 days) to be replicated; yet the entire human genome can be replicated within several hours. How is this possible?arrow_forward
- In the practical you have been analysing a human genomic library. You know from your calculations that only a small proportion of the human genome is represented, even when the entire class results are considered. Therefore, the chance of finding a particular single-copy gene in your library is very small. Outline a strategy for constructing a genomic DNA library more representative of the entire human genome. You will need to consider alternative vectors and the efficiency of transformation of the bacterial cells.arrow_forwardWhat is a plasmid? Why is it an ideal tool in replicating genetic sequences?arrow_forwardYou are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture of E. coli. After you run the experiment, you decide to use gel electrophoresis to genotype the different bacterial cultures to determine if the gene editing was successful. How could your electrophoresis results confirm that the PCR was successful? And how could your electrophoresis results confirm that you successfully extracted genomic DNA from your bacterial samples?arrow_forward
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