Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 11.6, Problem 1TYK
Which of the following components are needed for the adaptation phase of the CRISPR-Cas system?
- crRNA and Cas1
- crRNA and Cas2
- crRNA and Cas9
- Cas1 and Cas2
- tracrRNA and Cas9
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In the CRISPR-Cas system, what does the tracrRNA bind to?a. crRNA and Cas1b. crRNA and Cas2c. crRNA and Cas9d. Cas1 and Cas2
What is the function of gRNA in CRISPR-Cas technologies?It cuts DNA at a targeted sequence and inserts a new sequence -.It cuts protein at a targeted sequence and inserts a new sequence.It ensures the Cas9 enzyme cuts at the correct place in the DNA.It ensures the Cas9 enzyme cuts at the correct place in the RNA.It replaces uracil nucleotides with thymine nucleotides.
Name the components and the function of each of a CRISPR/Cas9 genetic editing system.
Chapter 11 Solutions
Principles of Biology
Ch. 11.1 - Which of these binding interactions might be...Ch. 11.1 - Prob. 1TYKCh. 11.1 - When an ncRNA functions as a decoy, it contains...Ch. 11.2 - Prob. 1BCCh. 11.2 - Prob. 1CCCh. 11.2 - Prob. 1TYKCh. 11.3 - Prob. 1CCCh. 11.3 - Prob. 1TYKCh. 11.4 - Explain why RISC binds to a specific mRNA. What...Ch. 11.4 - The process of RNA interference may lead to the...
Ch. 11.4 - To catalyze the methylation of an rRNA, a snoRNA...Ch. 11.5 - Prob. 1BCCh. 11.5 - Prob. 1TYKCh. 11.6 - Prob. 1CCCh. 11.6 - Which of the following components are needed for...Ch. 11.6 - Prob. 2TYKCh. 11.7 - Prob. 1TYKCh. 11 - Which of the following types of molecules could...Ch. 11 - Prob. 2TYCh. 11 - Prob. 3TYCh. 11 - Prob. 4TYCh. 11 - Prob. 5TYCh. 11 - Prob. 6TYCh. 11 - Prob. 7TYCh. 11 - Prob. 8TYCh. 11 - Prob. 9TYCh. 11 - Prob. 10TYCh. 11 - An ncRNA may have the following funtions:...Ch. 11 - Prob. 2CCQCh. 11 - Prob. 3CCQCh. 11 - Review the concept of an RNA world described in...Ch. 11 - Prob. 2CBQ
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- explain what CRISPR/cas13 is and how is it different from CRISPR/cas9, the gene editing toolsarrow_forwardCRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however, but instead adapted from a system that already exists in nature. Describe the native biological function of CRISPR-Cas9.arrow_forwardCRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the following component(s) of this system contribute to the specificity of the endonucleolytic cleavage Options: the sequence of the sgRNA the PAM sequence Cas9 a and b a, b and carrow_forward
- Which of the following statements is not true about the guide RNA (see this interactive demonstration for help with this question: https://www.biointeractive.org/classroom-resources/crispr-cas9-mechanism-applications) Group of answer choices it contains a sequence of 20 nucleotides that matches a specific sequence in a cell’s DNA When the guide RNA is combined with Cas9, it will guide Cas9 to the target sequence its target sequence can be almost any sequence as long as it occurs near a PAM motif it is a nuclease, a type of enzyme that cleaves DNAarrow_forwardWhat are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.arrow_forwardWhich of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer choices A single guide RNA (sgRNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence A single guide RNA (sgRNA) recognizes a genomic region followed by a long sequence palindrome repeat A single guide DNA (sgDNA) recognizes a genomic region followed by 5'-NGG-3' PAM sequence PAM sequences induce single stranded breaks that are then repaired by the CAS-9 enzymearrow_forward
- Explain How Single-nucleotide mutations can be introduced into the genome using an engineered CRISPR-Cas9 system.arrow_forwardList the components that you would need in order to use the CRISPR-Cas9 system for gene editingarrow_forwardWhere did the Crispr system first originate and what is its purpose in nature? What is the advantage to using the CRISPR-Cas9 system compared to previous gene editing technologies? What are the advantages? Any drawbacks? What are the ethicals concerns that are raised with the use and application of this technology? What do you think of this technology?arrow_forward
- Using figure (a) above as guide, list the five components in the CRISPR-Cas9 bacterial defense system used against bacteriophage infection. In 25 words or less describe the role of each component of the system. (1) (2) (3) (4) (5)arrow_forwardWhich of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.arrow_forwardWhich of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.arrow_forward
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