Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 12, Problem 6TY
Summary Introduction

Introduction:

In E.coli, lac operon is present that contains genes required for the metabolism of lactose. The lac operon is activated in the absence of glucose and presence of lactose. Two regulators required to turn on and off the operon are catabolite activator protein (CAP) and the lac repressor.

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When an individual drink's a product with lactose (e.g., whole milk), what happens to the repressor system of the lac operon? The lac repressor is inactivated Transcription of the lac operon genes into a single mRNA β-galactosidase and lac permease are synthesized Lactose is rapidly metabolized by enzymes
For the lac operon, determine whether genes will be expressed (on) or silenced (off) given the following: a. Lactose levels are high, glucose is low. b. The lac repressor can no longer bind DNA; lactose and glucose are low.
Describe  the regulation of the lac operon when lactose is present and glucose is present  Your Response must include the following terms, cAMP, inactive CAP, active CAP  promoter, operator, RNA polymerase, CAP binding site
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