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Cloned Libraries The steps in the polymerase chain reaction (PCR) are: a. breaking hydrogen bonds, annealing primers, and synthesizing using DNA polymerase b. restriction cutting, annealing primers, and synthesizing using DNA polymerase c. ligating, restriction cutting, and transforming into bacteria d. DNA sequencing and restriction cutting e. transcription and translation

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 13, Problem 15QP
Textbook Problem
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Cloned Libraries

The steps in the polymerase chain reaction (PCR) are:

  1. a. breaking hydrogen bonds, annealing primers, and synthesizing using DNA polymerase
  2. b. restriction cutting, annealing primers, and synthesizing using DNA polymerase
  3. c. ligating, restriction cutting, and transforming into bacteria
  4. d. DNA sequencing and restriction cutting
  5. e. transcription and translation

Summary Introduction

Introduction: A process of molecular biology which is responsible for the production of various copies of a particular segment of DNA is called “polymerase chain reaction (PCR).” Using the technique of PCR, a single segment of DNA can be amplified to produce sufficient number of copies of that particular segment.

Explanation of Solution

Reason for correct answer:

In polymerase chain reaction, the hydrogen bonds between the nucleotides are broken down with the help of heat. The addition of primers to the strand of DNA takes place which initiates the process of PCR. Furthermore, the nitrogenous bases start attaching to their complementary bases which are present on the DNA strand by the functioning of the polymerase enzyme.

Option a. is given as “breaking hydrogen bonds, annealing primers, and synthesizing using DNA polymerase.”

Polymerase chain reaction includes steps such as “breaking hydrogen bonds, annealing primers, and synthesizing using DNA polymerase.” Hence, the correct answer is option a.

Reason for incorrect answer:

Option b. is given as, “restriction cutting, annealing primers, and synthesizing using DNA polymerase.”

The process of annealing primers takes place after the breaking of hydrogen bonds between the complementary nucleotides...

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