a. To determine: The method of entry of a transposon into the bacteria and the identification of new insert. Introduction: A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

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Answer 1

Question

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

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Students have asked these similar questions

Xeroderma pigmentosum is a genetic disease caused by an error in the nucleotide excision repair process that fixes damage to DNA by ultraviolet light. Studies have shown that it can result from mutations in any one of seven genes. What can you infer from this finding? A) There are seven genes that produce the same protein B) These seven genes are the most easily damaged by ultraviolet light. C) There are seven enzymes involved in the nucleotide excision repair process. D) These mutations have resulted from translocation of gene segments.

With reference to the image below, discuss the process and principle involved for screening/selection of hosts (last stage of cloning) containing the intended recombinant plasmid. LacZ' = Gene for alpha-peptide of β-galactosidase.

A plasmid, pUC18, contains the ampicillin-resistance gene, the origin of replication, and the ß - gal gene, which codes for the B-galactosidase protein. This protein can break down the synthetic chemical X-gal, producing a blue product that stains the entire cell blue (but is harmless to the bacteria). At the beginning of the B-gal gene there are several unique restriction sites (some of them are shown in the diagram below). You wish to clone a 1.0-kb Xbal fragment into the pUC18 plasmid, so you cut the plasmid with Xbal and, after removing the enzyme, mix the Xbal-cut plasmid with the 1.0-kb fragment, ligate, and transform competent bacteria. Pati Xbal EcoRI B-gal A Amp ori Figure: pUC18 plasmid map (a) On what medium would you grow your transformed bacteria? (b) Do you expect the bacteria carrying plasmid pUC18 (without the insert) to be blue or white when grown in the presence of X-gal? Explain.

Answer 2

Question

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

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Answer 3

Question

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

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Answer 4

Question

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

Expert Solution & Answer

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Answer 5

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

Answer 6

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Answer 7

Chapter 13, Problem 27P

Summary Introduction

a.

To determine:

The method of entry of a transposon into the bacteria and the identification of new insert.

Introduction:

A plasmid consists of nucleotides like chromosomal DNA, but it is not present inside the nucleoid. The plasmid has mainly used a vector for transferring the part of the genome of one organism into another.

Answer 8

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Answer 9

Summary Introduction

b.

To determine:

The use of plasmid as a mutagen.

Introduction:

Plasmid fingerprinting is performed in many organisms like Escherichia coli, Salmonella, Campylobacter, and Pseudomonas strains and this technique has been proved to be more accurate than other phenotyping methods, for example, biotyping, antibiotic-resistance patterns, phage typing, and serotyping.

Answer 10

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

Answer 11

Summary Introduction

c.

To determine:

The identification of the affected gene that is defective in plaque formation.

Introduction:

Viruses can be defined as acellular organisms that are obligate intracellular parasites. Viruses lack any cellular organelles but have only genetic material and protein-lipid core. These organisms cannot replicate by themselves, so they require a host cellular machinery to reproduce.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 13 - Choose the phrase from the right column that best...Ch. 13 - Prob. 2P Ch. 13 - Now that the sequence of the entire E. coli K12...Ch. 13 - Bacterial genomes such as that of E. coli...Ch. 13 - List at least three features of eukaryotic genomes...Ch. 13 - Describe a mechanism by which a gene could move...Ch. 13 - High salt concentrations tend to cause protein...Ch. 13 - Prob. 8P Ch. 13 - A liquid culture of E. coli at a concentration of...Ch. 13 - Pick out the medium i, ii, iii, or iv onto which...

Solution Summary: The author explains how a plasmid can be used to identify the affected gene. Plasmid fingerprinting is performed in many organisms.

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