a. To determine: The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum. Introduction: Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

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Answer 1

Question

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

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Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result? A) The colonies may be due to back mutation (reversion). B) The colonies may be due to recombination. C) Either A or B is possible. D) Neither A nor B is possible.

A bacterial transformation is performed with a donor strain that is resistant to four drugs, A, B, C, and D, and a recipient strain that is sensitive to all four drugs. The resulting recipient cell population is divided and plated onmedia containing various combinations of the drugs. The following table shows the results.a. One of the genes is distant from the other three, which appear to be closely linked. Which is the distant gene? b. What is the likely order of the three closely linked genes

All of the following apply to Luria and Delbrűck’s mutation theory as tested using E. Coli and T1 phage EXCEPT: A. It was performed using liquid bacterial cultures B. It involved looking for T1-resistant bacteria as an end measure C. It was supported by the finding that the number of final cultures that were T1 phage-resistant were constant from experiment to experiment D. It rejected the possibility of mutations occurring only in response to a T1 phage challenge E. It supported the concept of spontaneous mutation rates

Answer 2

Question

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

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Answer 3

Question

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

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Answer 4

Question

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

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Answer 5

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

Answer 6

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Answer 7

Chapter 13, Problem 11P

Summary Introduction

a.

To determine:

The significance of ensuring that reversion does not take place in the experiment of Lederberg and Tatum.

Introduction:

Reversion, also known as the reverse mutation, is the mutation process in which the wild type genome is restored. The organisms that show reversion are known as reverants. The bacteria exhibits reversion, but it is a very rare event.

Answer 8

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

Answer 9

Summary Introduction

b.

To determine:

The modification in the experiment to indicate the donor and recipient bacteria during conjugation.

Introduction:

There are three modes of transfer of genetic material that takes place in the bacteria. The processes by which the horizontal gene transfer takes place are categorized as transformation, transduction, and conjugation. The change in the genetic composition can also be brought about by mutation.

Answer 10

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Answer 11

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Answer 12

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Answer 13

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Solution Summary: The author explains that Lederberg and Tatum's experiment did not show which bacteria was a donor and which was recipient in the process of conjugation.

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