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Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 13, Problem 6P
Describe a mechanism by which a gene could move from the bacterial genome to a plasmid in the same cell, or
vice versa.
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explain how Plasmids Carry Genes in Addition toThose in the Bacterial Chromosome
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Chapter 13 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 13 - Choose the phrase from the right column that best...Ch. 13 - Prob. 2PCh. 13 - Now that the sequence of the entire E. coli K12...Ch. 13 - Bacterial genomes such as that of E. coli...Ch. 13 - List at least three features of eukaryotic genomes...Ch. 13 - Describe a mechanism by which a gene could move...Ch. 13 - High salt concentrations tend to cause protein...Ch. 13 - Prob. 8PCh. 13 - A liquid culture of E. coli at a concentration of...Ch. 13 - Pick out the medium i, ii, iii, or iv onto which...
Ch. 13 - Prob. 11PCh. 13 - In two isolates one is resistant to ampicillin,...Ch. 13 - E. coli cells usually have only one copy of the F...Ch. 13 - In E. coli, the genes purC and pyrB are located...Ch. 13 - DNA sequencing of the entire H. influenzae genome...Ch. 13 - Genes encoding toxins are often located on...Ch. 13 - a. You want to perform an interrupted-mating...Ch. 13 - Prob. 18PCh. 13 - One issue with interrupted-mating experiments such...Ch. 13 - Prob. 20PCh. 13 - Starting with an F- strain that was prototrophic...Ch. 13 - You can carry out matings between an Hfr and F...Ch. 13 - Genome sequences show that some pathogenic...Ch. 13 - Generalized and specialized transduction both...Ch. 13 - Prob. 25PCh. 13 - Prob. 26PCh. 13 - Prob. 27PCh. 13 - The sequence at one end of one strand of the...Ch. 13 - Prob. 29PCh. 13 - Suppose that you could obtain radioactively...
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- The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for α-globin into a bacterial plasmid and have the bacteria produce α-globin?arrow_forwardList three important characteristics of plasmids and their functionarrow_forwardBacterial conjugation has medical consequences. For example, certain plasmids contain genes that code for toxins. The causative agent of a deadly form of food poisoning, E. coli 0157, synthesizes a toxin that causes massive bloody diarrhea and kidney failure. This toxin is now believed to have originated in Shigella, another bacterium that causes dysentery. Similarly, the growing problem of antibiotic resistance is partly attributable to the spread of antibioticresistant genes among bacterial populations. Antibiotic resistance develops because antibiotics are overused in medical practice and in livestock feeds. Suggest a mechanism by which this extensive use promotes antibiotic resistance.arrow_forward
- Explain, with the aid of a hand drawn diagram, the life cycle of bacteriophage T4.arrow_forwardExplain the basis for the following statement: Transcription of two genes on a plasmid can occur without the concomitant action of a topoisomerase, but only if those two genes are oriented in opposite directions.arrow_forwardoutline the sequence of events that comprise the mechanism used by a bacteriophage in order to infect a bacteria cell.arrow_forward
- Outline the sequence of events that compromise the mechanism used by a bacteriophage in order to infect a bacteria cell?arrow_forwardWhich of the following is NOT a mechanism by which plasmids inerease the possibility of being present in the new generation of cells? A) O They can be present at a high number so that some copies will end up in each new cell after cell division. B) O They encode traits such as antibiotic resistance that are required for growth in some environments. C) O They cause the cell to replicate much more rapidly than cells that do not have plasmids. D) O They may carry host survival genes and self-preservation genes.arrow_forwardIf we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forward
- In which of the four processes shown can material from a bacterial genome be transferred from one cell to another? Which are forms of horizontal transmission? Place the answer(s) in the cell highlighting that process. Partial genome transfer by DNA uptake Transformation Answer Bank material from a bacterial genome horizontal gene transfer Conjugation Plasmids Conjugation Genome Plasmid transfer during conjugation Partial genome transfer during conjugation Virus Transduction Transfer as part of viral genomearrow_forwardWhich of the following statements is true of plasmids? Agrobacteria use plasmids to induce tumnors in plant genomes Scientists use plasmids to introduce recombinant DNA into plants and animals They are parasitic DNA that replicate in human cells. Bacteria are transformed when they take plasmids from their extra cellular environment They are circular fragments of DNA that naturally occur in bacteria Bt corn expresses a toxin encoded on a plasmidarrow_forwardA hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forward
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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license