EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 13, Problem 3Q
Summary Introduction
To review:
Whether deoxyribonucleic acid (
Given:
In an experiment, a pulse and chase method were applied to the cells and the DNA extracted from them was subjected to the gel electrophoresis.
Figure 1: DNA bands representing a pulse and chase method.
Introduction:
The duplication of DNA takes place during the S phase of the cell cycle. If the cells are subjected to the medium containing radioactive thymidine during this phase, then the DNA extracted from them can be observed in the gel electrophoresis.
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Below is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers
(1) Which end (5' or 3') of the molecule is here?
(2) Which enzyme is probably functioning here to deal with supercoils in the DNA?
(3) Which enzyme is probably functioning here to unwind the DNA?
If DNA is replicating in a cell, how does it do this? Describe the mechanism of
replication.
Is the semiconservative replication of DNA perfect?
Chapter 13 Solutions
EBK LIFE: THE SCIENCE OF BIOLOGY
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- DNA replication is vital for successful cell division. Explain the process of DNA replication. Make sure to use the following terms: helicase, S-phase, DNA polymerase and DNA ligase, template, free nucleotides. Use the diagram if it helps you illustrate your points.arrow_forwardWhy is DNA replication is considered a semi-discontinuous process? Explain in detail.arrow_forwardBelow is a diagram of DNA replication as currently believed to occur in E. coli. From specific points, arrows are provided that lead to numbers. Answer the questions below relating to the locations specified by the numbers. (02) What end (5’ or 3’) of the molecule is here? (State which) What enzyme is probably functioning here to deal with supercoils in the DNA? What enzyme is probably functioning here to unwind the DNA? What nucleic acid is probably depicted here? What are these short DNA fragments usually called? What enzyme probably functions here to couple these two newly synthesized fragments of DNA? Is this strand the leading or lagging strand? What end (5’ or 3’) of the molecule is here? (State which)arrow_forward
- Why are enzymes needed in the process of the DNA replicationarrow_forwardWhat is the function of Gyrase during DNA Replicationarrow_forwardGive only typing answer with explanation and conclusion why is DNA replication semi-conservative? like how is this process discontinuous and continuous?arrow_forward
- If ligase was defective in a cell, which strand of DNA Replication do you think would be most affected, the leading or lagging strand? Fully explain why you think that by describing the enzyme function and the differences in replication in the leading and lagging strands.arrow_forwardDNA polymerases have a shape resembling a right hand with three functional domains. What are the roles of these domains in DNA replication?arrow_forwardIn NOT more than 200 words, explain how the double-helical structure of DNA suggests a mechanism for DNA replication?arrow_forward
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