EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 13.3, Problem 1R
Summary Introduction
To review:
Meselson-Stahl experiment differentiating between three models of
Introduction:
Meselson-Stahl experiment was proposed by two scientists called Matthew Meselson and Franklin Stahl. In 1958, they supported Watson and Crick experiment which stated replication of genetic material called DNA (deoxyribonucleic acid) is semiconservative.
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What did the Watson–Crick model suggest about the replication of DNA?
How many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?
If DNA replication followed the dispersive model of replication, how would the outcomes of the Meselson-Stahl experiment change? Describe the composition of DNA samples after one and two rounds of replication, and how this is different from the findings of the original experiment.
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EBK LIFE: THE SCIENCE OF BIOLOGY
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- Why were Meselson and Stahl unable to distinguish between the dispersive and the semiconservative models following only one round of DNA replication?arrow_forwardWhat is meant by semi conservative replication? How did Meselson and Stahl prove it experimentally?arrow_forwardIn the Meselson–Stahl experiment, which of the three modes of replication could be ruled out after one round of replication? after two rounds?arrow_forward
- Does the design of the Hershey–Chase experiment distinguish between DNA and RNA as the molecule serving as the genetic material? Why or why not?arrow_forwardDescribe the Meselon -Stahl experiment Include how they distinguished new from old DNA strands, what the centrifuge was for, and how it showed semi-conservative replication.arrow_forwardWhat are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.arrow_forward
- In the Meselson-Stahl experiment on DNA replication, what fraction of the DNA was composed of one light strand and one heavy strand ("hybrid") after one generation of growth in medium containing 14N? After two generations of growth in a medium containing 14N? What fraction of hybrid DNA is expected after n generations of growth in a medium containing 14N?arrow_forwardWhat result would Meselson and Stahl have obtained if the replication of DNA were conservative (i.e., the parental double helix stayed together)? Give the expected distribution of DNA molecules after 1.0 and 2.0 generations for conservative replication.arrow_forwardIn the Meselson and Stahl experiment, what part of the DNA gets labeled with 15N? Why?arrow_forward
- Who experimentally prove that DNA replication is semi conservative?arrow_forwardShow the watson-crick model for semoconservative DNA replication?arrow_forwardConsider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.arrow_forward
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