Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 13, Problem 3Q
Summary Introduction
To review:
Whether deoxyribonucleic acid (
Given:
In an experiment, a pulse and chase method were applied to the cells and the DNA extracted from them was subjected to the gel electrophoresis.
Figure 1: DNA bands representing a pulse and chase method.
Introduction:
The duplication of DNA takes place during the S phase of the cell cycle. If the cells are subjected to the medium containing radioactive thymidine during this phase, then the DNA extracted from them can be observed in the gel electrophoresis.
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Chapter 13 Solutions
Life: The Science of Biology
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- “DNA polymerase plays a dual function during DNA replication” comment onstatement?arrow_forwardWhich pattern does the structures of dna follow in that its replication results in one parent molecule and one daughter molecule.arrow_forwardIn the process of DNA replication, there are four important enzymes. Construct a table, provide what the four enzymes are, and explain how they function in DNA replication.arrow_forward
- Identify the site of protein synthesis in a cell. Is the site for protein synthesis the same as that for DNA replication? Justify your answer and explain why this is necessary.arrow_forwardGive only typing answer with explanation and conclusion why is DNA replication semi-conservative? like how is this process discontinuous and continuous?arrow_forwardIf ligase was defective in a cell, which strand of DNA Replication do you think would be most affected, the leading or lagging strand? Fully explain why you think that by describing the enzyme function and the differences in replication in the leading and lagging strands.arrow_forward
- Explain how the double helical structure of the DNA suggests a mechanism for DNA replicationarrow_forwardAnalyze the experiment of Meselson and Stahl and explain how the results were consistent with the semiconservative model of DNA replication.arrow_forwardSuppose strong phosphodiester bonds were found between the bases on opposite strands of a DNA molecule, and weak H-bonds were found between the nucleotides within each polynucleotide strand. Would DNA replication work as before? Why or why not?arrow_forward
- Is the semiconservative replication of DNA perfect?arrow_forwardCompare and contrast the activity of a topoisomerase and a helicase in the context of DNA replication. Answer in 2-3 sentences.arrow_forwardWhich describes the role of primase during replication? a. It catalyzes the formation of phosphodiester bonds using NTPs as substrates. \ b. It coordinates synthesis of the leading strand and the lagging strand. c. It functions as a holoenzyme that polymerizes in the 3’→ 5’ direction. d. It uses an exonuclease activity to remove incorrect nucleotides.arrow_forward
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