Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 16P
a. Calculate the standard free energy change as a pair of electrons is transferred from succinate to molecular oxygen in the mitochondrial respiratory chain.
b. Based on your answer in part a, calculate the maximum number of protons that could be pumped out of the matrix into the intermembrane space as these electrons are passed to oxygen. Assume 25o C, ΔpH = 1.4;
c. At which site(s) are these protons pumped?
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1 a) What is meant by the ATP currency exchange ratio? Why does the oxidation of mitochondrial FADH2 generate one less ATP than oxidation of mitochondrial NADH?
b) If 12 H+ are moved across the inner mitochondrial membrane by NADH oxidation, and each ATP synthesized requires 3 H+ to move through ATP synthase, why are only 3 ATP molecules produced by oxidation of each NADH?
For the mitochondrial membrane described in pic, how many protons must be transported to provide enough free energy for the synthesis of 1 mol of ATP (assuming standard biochemical conditions)?
Calculate how many moles of ATP can be made in standard conditions when 1 mole of protons flow back into the mitochondrial matrix through the ATP synthase. Assume that: i) the matrix pH is 7.8, ii) the cytosolic pH is 7.2, iii)ΔE=0.168 V (inside negative), iv) T=37°C, and v) the standard free energy change for the hydrolysis of 1 mole of ATP is -31.3 kJ/mol.
Chapter 14 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 14 - Prob. 1PCh. 14 - When pure reduced cytochrome c is added to...Ch. 14 - Freshly prepared mitochondria were incubated with...Ch. 14 - Prob. 4PCh. 14 - Prob. 5PCh. 14 - Prob. 6PCh. 14 - Intramitochondrial ATP concentrations are about 5...Ch. 14 - Prob. 8PCh. 14 - Prob. 9PCh. 14 - Years ago there was interest in using uncouplers...
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