The steps of biochemical pathway showing the sequence of different compounds and enzymes on the basis of the given data. Given: Bacterial strains mutated for five different enzymes (1, 2, 3, 4, and 5) were grown on a variety of media, which was substituted with different compounds involved in the pathway. Table 1 represents the mutant strain growth on the medium having different compounds required in the biochemical pathway. Table 1: Growth of different mutants on medium containing different compounds of the biochemical pathway. In the table shown above, compound ‘C’ or chorismate is the precursor in the biochemical pathway and ‘T’ or tryptophan is the end product. Compound D, E, F, and G are intermediates. The ‘+’ represents growth when the indicated compound was added to the growth medium, and ‘0’ represents the absence of growth. Introduction: In a wild-type bacterium, the synthesis of tryptophan takes place through a series of enzymatic reactions starting from the precursor, chorismate. In this experiment, to deduce the biochemical pathway, the bacterial strains have been mutated for different enzymes and the growth of the bacteria in presence of different compounds has been recorded.

Question

Want to see the full answer?

Answer 1

Question

Chapter 14.1, Problem 3R

Summary Introduction

To analyze:

The steps of biochemical pathway showing the sequence of different compounds and enzymes on the basis of the given data.

Given:

Bacterial strains mutated for five different enzymes (1, 2, 3, 4, and 5) were grown on a variety of media, which was substituted with different compounds involved in the pathway. Table 1 represents the mutant strain growth on the medium having different compounds required in the biochemical pathway.

Table 1: Growth of different mutants on medium containing different compounds of the biochemical pathway.

In the table shown above, compound ‘C’ or chorismate is the precursor in the biochemical pathway and ‘T’ or tryptophan is the end product. Compound D, E, F, and G are intermediates. The ‘+’ represents growth when the indicated compound was added to the growth medium, and ‘0’ represents the absence of growth.

Introduction:

In a wild-type bacterium, the synthesis of tryptophan takes place through a series of enzymatic reactions starting from the precursor, chorismate. In this experiment, to deduce the biochemical pathway, the bacterial strains have been mutated for different enzymes and the growth of the bacteria in presence of different compounds has been recorded.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

The intermediates A, B, C, D, E, and F all occur in the same biochemical pathway G is the product of the pathway, and mutations 1 through 7 are all G –, meaning that they cannot produce substance G. The following table shows which intermediates will promote growth in each of the mutants. Arrange the intermediates in order of their occurrence in the pathway at which each mutant strain is blocked. A “+” in the table indicates that the strain will grow if given that substance, an “o” means lack of growth.

In a line of human cells grown in culture, a geneticist isolates a temperature-sensitive mutation at a locus that encodes an acetyltransferase enzyme; at temperatures above 38°C, the mutant cells produce a nonfunctional form of the enzyme. What would be the most likely effect of this mutation if the cells were grown at 40°C?

The reason why Beadle and Tatum observed four different categories of mutants that could not grow on media without methionine is because a. the enzyme involved in methionine biosynthesis is composed of four different subunits. b. the enzyme involved in methionine biosynthesis is present in four copies in the Neurospora genome. c. four different enzymes are involved in a pathway for methionine biosynthesis. d. a lack of methionine biosynthesis can inhibit Neurospora growth in four different ways.