# (a) Interpretation: The calibration curve should be constructed in a spread sheet. Concept introduction: A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations. ### Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213 ### Principles of Instrumental Analysis

7th Edition
Douglas A. Skoog + 2 others
Publisher: Cengage Learning
ISBN: 9781305577213

#### Solutions

Chapter 15, Problem 15.7QAP
Interpretation Introduction

## (a)Interpretation:The calibration curve should be constructed in a spread sheet.Concept introduction:A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations.

Interpretation Introduction

### (b)Interpretation:The least square slope and the intercept for the plot in (a) should be determined.Concept introduction:Syy=∑(yi−y¯)2Sxx=∑(xi−x¯)2Sxy=∑(xi−x¯)(yi−y¯)The slope of the line, m=SxySxxThe intercept, b=y¯−mx¯

Interpretation Introduction

### (c)Interpretation:The standard deviation of the slope and the standard deviation about regression for the curve should be determined.Concept introduction:Standard deviation about regression, sr=Syy−m2SxxN−2N − number of points used.The standard deviation of the slope, sm=sr2SxxThe standard deviation of the intercept, sb=sr∑xi2N∑xi2−(∑xi)2

Interpretation Introduction

### (d)Interpretation:The concentration of unknown NADH sample should be calculated using the spreadsheet.Concept introduction:A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations.

Interpretation Introduction

### (e)Interpretation:The relative standard deviation for the result in part (d) should be calculated.Concept introduction:Standard deviation of the results obtained from the calibration curve = sc=srm1M+1N+(y¯c−y¯)2m2SxxM − number of replicatesN- number of calibration points.RSD=sx×100RSD − relative standard deviationx - mean of x valuess − standard deviation

Interpretation Introduction

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