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ChemistryPrinciples of Instrumental Analysis(a) Interpretation: The calibration curve should be constructed in a spread sheet. Concept introduction: A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations.Start your trial now! First week only $4.99!*arrow_forward*

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7th Edition

Douglas A. Skoog + 2 others

Publisher: Cengage Learning

ISBN: 9781305577213

Chapter 15, Problem 15.7QAP

Interpretation Introduction

**(a)**

**Interpretation:**

The calibration curve should be constructed in a spread sheet.

**Concept introduction:**

A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations.

Interpretation Introduction

**(b)**

**Interpretation:**

The least square slope and the intercept for the plot in (a) should be determined.

**Concept introduction:**

The slope of the line,

The intercept,

Interpretation Introduction

**(c)**

**Interpretation:**

The standard deviation of the slope and the standard deviation about regression for the curve should be determined.

**Concept introduction:**

Standard deviation about regression,

N − number of points used.

The standard deviation of the slope,

The standard deviation of the intercept,

Interpretation Introduction

**(d)**

**Interpretation:**

The concentration of unknown NADH sample should be calculated using the spreadsheet.

**Concept introduction:**

A calibration curve or standard curve is a method used in analytical chemistry to determine the concentration of unknown sample by comparing to a set of samples with known concentrations.

Interpretation Introduction

**(e)**

**Interpretation:**

The relative standard deviation for the result in part (d) should be calculated.

**Concept introduction:**

Standard deviation of the results obtained from the calibration curve =

M − number of replicates

N- number of calibration points.

RSD − relative standard deviation

x - mean of x values

s − standard deviation

Interpretation Introduction

**(f)**

**Interpretation:**

The relative standard deviation for the result in part (d) should be calculated if a result of 7.95 was the mean of three measurements.

**Concept introduction:**

Standard deviation of the results obtained from the calibration curve =

M − number of replicates

N- number of calibration points.

RSD − relative standard deviation

x - mean of x values

s − standard deviation