Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
3rd Edition
ISBN: 9780134832258
Author: Mark F. Sanders, John L. Bowman, Peter Mirabito
Publisher: PEARSON
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Chapter 15, Problem 33P
Describe how having the Cas
Figure 15.23
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A bidirectional enhancer has the following sequence:
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Chapter 15 Solutions
Study Guide And Solutions Manual For Genetic Analysis: An Integrated Approach
Ch. 15 - 15.1 What purpose do the bla and lacZ genes serve...Ch. 15 - The human genome is 3109 bp in length. How many...Ch. 15 - 15.3 Ligase catalyzes a reaction between the...Ch. 15 - You have constructed four different libraries: a...Ch. 15 - Using the genomic libraries in Problem 4, you wish...Ch. 15 - The human genome is 3109bp. You wish to design a...Ch. 15 - 15.7 Using animal models of human diseases can...Ch. 15 - 15.8 Compare methods for constructing homologous...Ch. 15 - 15.9 Chimeric genefusion products can be used for...Ch. 15 - 15.10 Why are diseases of the blood simpler...
Ch. 15 - Injection of double-stranded RNA can lead to gene...Ch. 15 - Compare and contrast methods for making transgenic...Ch. 15 - 15.13 It is often desirable to insert cDNAs into a...Ch. 15 - 15.14 A major advance in the s was the development...Ch. 15 - 15.15 The bacteriophage lambda genome can exist in...Ch. 15 - 15.16 The restriction enzymes Xho and Sal cut...Ch. 15 - 15.17 The bacteriophage has a single-stranded DNA...Ch. 15 - 15.18 To further analyze the CRABS CLAW gene (see...Ch. 15 - You have isolated a genomic clone with an EcoR I...Ch. 15 - 15.20 You have identified a cDNA clone that...Ch. 15 - 15.21 You have isolated another cDNA clone of the...Ch. 15 - 15.22 You have identified five genes in S....Ch. 15 - You have generated three transgenic lines of maize...Ch. 15 - 15.24 Bacterial Pseudomonas species often possess...Ch. 15 - 15.25 Two complaints about some transgenic plants...Ch. 15 - 15.26 In Drosophila, lossoffunction Ultrabithorax...Ch. 15 - Prob. 27PCh. 15 - The highlighted sequence shown below is the one...Ch. 15 - Vitamin E is the name for a set of chemically...Ch. 15 - The RAS gene encodes a signaling protein that...Ch. 15 - 15.31 You have cloned a gene for an enzyme that...Ch. 15 - 15.32 About of occurrences of nonautoimmune type...Ch. 15 - Describe how having the Cas 9 gene at a genomic...Ch. 15 - 15.34 Would a gene drive system spread rapidly...
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- Rsearcher has discovered that one human promoter is responsible for producing two different proteins (Protein K and Protein B) from the same gene. In Kidney cells it is responsible for the production of Protein K, while in Brain cells it is responsible for the production of Protein B. How are these different proteins being produced from the same gene? Describe the mechanismarrow_forwardDescribe a ChIP-Seq experiment that will enable the identification of promoters to which the stationary phase sigma factor RpoS binds in E. coli. How could you also determine when RpoS binds to DNA?arrow_forwardYou then make a screen to identify potential mutants (shown as * in the diagram) that are able to constitutively activate Up Late operon in the absence of Red Bull and those that are not able to facilitate E. Coli growth even when fed Red Bull. You find that each class of mutations localize separately to two separate regions. For those mutations that prevent growth even when fed Red Bull are all clustered upstream of the core promoter around -50 bp. For those mutations that are able to constitutively activate the operon in the absence of Red Bull are all located between the coding region of sleep and wings. Further analysis of each DNA sequence shows that the sequence upstream of the promoter binds the protein wings and the region between the coding sequence of sleep and wings binds the protein sleep. When the DNA sequence of each is mutated, the ability to bind DNA is lost. Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the Up Late…arrow_forward
- i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion protein. Please describe the strategy that you will follow to edit this gene with CRISPR-Cas9 technology, in order to visualize the expression of gene X in live cells. (ii) Please explain how a knockout mouse for a gene X can be produced with CRISPR-Cas9 technologyarrow_forwardBriefly describe how the transcript beginning at the lambda PRe promoter can inhibit expression of the cro gene.arrow_forwardDesign a six-residue nucleic acid probe that would hybridize with the greatest number of E. coli gene promoters.arrow_forward
- Suppose you had a bacteriophage λ in which the gene encoding the cro protein had been mutated, so that it had an unusually high affinity for pL - in this phage the first promoter repressed by cro would be pL, not pRM. What effect, if any, would this mutation, and the consequent rapid repression of transcription from pL, have on the outcome of a cell being infected by this mutant bacteriophage? Explain why you would predict that outcome of aninfection by this mutant phage.arrow_forwardYou manipulate the cro and cI/lambda repressor proteins so that they end up with swapped DNA binding domains (cI now has cro’s DNA binding domain, and cro now has cIs). This mutant bacteriophage then infects a happy, healthy population of bacterial cells. How will this modified cro protein interact with the three OR sites, and how would cro expression be affected? Include a drawing if possible.arrow_forwardExplain the basis of observation and way to test the hypothesis for the insertion of B-galactosidase lacking its own transcription control inserted in a cluster of piRNA genes inhibits normal expression of gene elsewhere in the genome and when inserted outside the piRNA gene cluster, the normal gene is expressed properly.arrow_forward
- Locate a gene expression data set on Gene Expression Omnibus using "expression by hybridization" involving Rabies virus or related virus Use GEO2R to perform pairwise group analysis Explore and identify the most regulated genes: gene/protein functions of Rabies virusand Define the significance of these genes/proteins in the disease process.arrow_forwardExplain the CRISPR-Cas Mechanism for RNA-Guided Destruction of Invading DNA ?arrow_forwardA high rate of transcription initiation from promoters in bacteria can be achieved by which of the following? a. the presence of perfect consensus sequences at the -35 and -10 positions of the promoter. b. the binding of an activator protein close to the promoter. c. the presence of an UP element immediately upstream of the promoter. d. a and c, but not b e. a, b, and carrow_forward
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