Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 15.3, Problem 1C
Explain how you would compare the expression of a gene in a cancer cell and a normal cell using qPCR, a DNA chip, and RNA-seq analysis.
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Discuss how qPCR, DNA microarrays (DNA chips), and RNA- seq analysis are used to compare levels of gene expression among normal versus diseased tissues.
In a microarray experiment, if you label cDNA from Tissue #1 with a red color and cDNA from tissue #2 with a green color and perform a microarray, predict the following gene expression patterns? :
A dot that is only red?
A dot that is only green?
A dot that is only yellow?
A dot that is black?
How is DNA microarray technology used to determine the differences in gene expression?
Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.arrow_forwardWhat gene expression question could you answer using data from the ENCODE website?arrow_forwardGive a step by step method and diagram of how reversible terminator sequencing works.arrow_forward
- Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for effective precision medicine?arrow_forwardExplain how you might use GenBank to obtain information about the function of a gene that you cloned and sequenced from a tumor cell.arrow_forwardExplain Gene Expression Microarrays and RNA-seq in Transcriptome Analysis as detailed and thoroughly as you can.arrow_forward
- What is the difference between manipulating a gene for gene therapy and for producing a transgenic organism?arrow_forwardWould gene chips containing bacterial DNA segments be useful for monitoring gene expression in a mammalian cell?arrow_forwardFrom microarray analysis how do we know what genes are being expressed in a specific tissue?arrow_forward
- Using a flow diagram, elaborate on how you would generate a recombinant plasmid that can be used for the expression of a therapeutic insulin.arrow_forwardSuppose you had funding to do a genome-wide gene expression experiment (20,000 genes). You can use a total of 20 RNA samples. Briefly describe the experiment you would run. In the context of your experiment, what is a false positive? In the context of your experiment, what is a false negativearrow_forwardWhat is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?arrow_forward
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