To analyze:
The two-hybrid system was used to identify interactions between proteins, and the succeeding results were obtained:
A clone coding gene A gave affirmative results with clones B and C, while clone B gave affirmative results with clones A, D, and E but not C, and clone E gave positive results only with clone B. Alternative clone F gave helpful results with clone G but not with any of
Provide the explaination of these results. Determine the approach by which two-hybrid system and genetic interaction results complement one another. whether reconcile two-hybrid system and genetic interaction results in a single model can reconcile.
Introduction:
The two- hybrid system is a method used to determine the interaction between two proteins. The system is based on the modular nature of the transcription factor
For the Two-Hybrid system, the transcription factor distributes into two domains i.e. DNA binding domain (DBD or BD) and activating domain (AD). Two fusion proteins are systematized
Both BD and AD have different functions. BD domain boosts the binding to UAS, while AD is responsible for the activation of the transcription.
Want to see the full answer?
Check out a sample textbook solutionChapter 16 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
- You are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from cheek cells of different people and amplified by PCR. When viewing the bands on the electrophoresis gel, one would expect that a taster (homozygote) would have---------band(s), whereas a carrier (heterozygote) would show--------band(s), and a non-taster would show------band(s).arrow_forwardIn a genomic analysis looking for a specific disease gene,one candidate gene was found to have a single-base-pairsubstitution resulting in a nonsynonymous amino acidchange. What would you have to check before concluding that you had identified the disease-causing gene?arrow_forwardLet’s assume the linker region of DNA averages 54 bp in length. How many molecules of H2A would you expect to find in a DNA sample that is 46,000 bp in length?arrow_forward
- Based on the alignment of these protein sequences, which [pairs] of the genes appear to be most similar to each other? And give reasons why?arrow_forwardThe genomes of two E. coli strains are compared in Figure14-19. Would you expect any third strain to contain moreof the blue, tan, or red regions shown in Figure 14-19?Explain.arrow_forwardGiven the Percentage Composition of One Nucleotide ina Genome, Can We Predict the Percentages of the OtherThree Nucleotides?arrow_forward
- You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located? Group of answer choices At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interestarrow_forwardOne experiment that showed the DNA carried genetic information was the "Transformation" experiment by Avery, McCarty, and MacLeod where they injected mice with non-pathogenetic bacteria that had been incubated in the remains of pathogenic bacteria. What did they find when they incubated non-pathogenic bacteria in just the protein from the pathogenic bacteria? In this case there was no transformation They found that the non-pathogenic bacteria were transformed into pathogenic bacteria In this case the mice were sickened but did not die They found that some of the protein could cause transformation but other proteins could notarrow_forwardIn the actual experiment, the researchers used 149 sequences to buildtheir sequence logo, which is shown below. There is a stack at eachposition, even if short, because the sequence logo includes moredata. (a) Which three positions in this sequence logo have the mostpredictable bases? Name the most frequent base at each. (b) Whichfour positions have the least predictable bases? How can you tell?arrow_forward
- What would be a primer sequence synthesized from the following DNA sequence: 5'-ACGTG-3'? out of 5'-GTGCA-3', 5'-CACGT-3', 5'-CACGU-3', 5'-TGCAC-3', or 5'-ACGTG-3'?arrow_forwardSuppose you are a research assistant in a lab studying dna-binding proteins. you have been given the amino acid sequences of all the proteins encoded by the genome of a certain species and have been asked to find candidate proteins that could bind dna. what type of amino acids would you expect to see in the dna-binding regions of such proteins?arrow_forwardDuring your experiment you analysed only a few of the recombinant clones for the presence of the highly repeated Aluelements. If you wanted to screen for a single-copy gene, you would need to screen a much larger genomic library. Assuming, that you already know the amino acid sequence of unicorn (a species with a similar physiology to humans) insulin, how would you construct a probe which would enable you to use nucleic acid hybridisation to screen a unicorn genomic DNA library for the insulin gene? Hint: you have access to any molecular biology reagents and equipment you might need, such as vectors, enzymes, and DNA sequencers.arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education