Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 16, Problem 32P
Great variation exists in the mechanisms by which RNAs can mediate gene regulation. In one recently discovered example shown in the following diagram, the genes CsrA and CsrB are global regulators of suites of target genes that are involved in the use of carbon atoms. The product of CsrA is the CsrA protein, which binds to the ribosome binding site (RBS) of target gene mRNA, preventing target gene expression. The product of the CsrB
gene is the CsrB RNA, which contains 22 binding sites for CsrA protein. CsrB RNA can thus compete with target mRNAs for CsrA protein binding. In the presence of high CsrB RNA concentrations, CsrA protein cannot bind to mRNA binding sites, so expression of the target genes is turned on.
| a. For the CsrA and CsrB genes, indicate what kind of molecule the gene product is (DNA, RNA, protein, small molecule), whether it acts as a positive or negative regulator, what stage of gene expression it affects, and whether it acts in cis or in trans. (It will be interesting to compare your answers to those for Problem 30.) |
| b. CsrA/CsrB regulate glycogen biosynthesis and breakdown; glycogen is a To what environmental factor do you think that the CsrA/CsrB system is most likely to respond? Suggest a possible way that this system might be repressible, and then suggest a different hypothesis for how this system might be inducible. (Assume in both cases that CsrB expression is modulated.) Which of your hypotheses would be most consistent with the target genes being involved in glycogen biosynthesis (an anabolic pathway), and which is most consistent with glycogen breakdown (a catabolic pathway)? Explain. |
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Chapter 16 Solutions
Genetics: From Genes to Genomes
Ch. 16 - For each of the terms in the left column, choose...Ch. 16 - The following statement occurs early in this...Ch. 16 - One of the main lessons of this chapter is that...Ch. 16 - All mutations that abolish function of the Rho...Ch. 16 - The figure at the beginning of this chapter shows...Ch. 16 - The promoter of an operon is the site to which RNA...Ch. 16 - You are studying an operon containing three genes...Ch. 16 - You have isolated a protein that binds to DNA in...Ch. 16 - You have isolated two different mutants reg1 and...Ch. 16 - Bacteriophage , after infecting a cell, can...
Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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