Genetics: From Genes to Genomes
Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 16, Problem 38P
Summary Introduction

a.

To determine:

The number of genes and operons depicted in the figure, the average gene density, and the representativeness of the value to the most bacterial genomes.

Introduction:

The mentioned figure shows the RNA-Seq results from the RNA-Seq experiment. In the analysis part, the computer lines up each of cDNA sequence read with the genome sequence of the bacterium.

Summary Introduction

b.

To determine:

Whether the given data proves that t2110 is nonfunctional and the process by which scientists were able to assign a direction to the transcription when no transcript of the gene was found.

Introduction:

In the performed experiment, it was found that no transcripts for the gene t2110 were detected.

Summary Introduction

c.

To determine:

The evidence that suggests the existence of an attenuation or riboswitch mechanism, causing premature transcriptional termination of an operon and the observation of any of the operon that might be controlled by such a mechanism.

Introduction:

The process of attenuation is considered as the method of control in some bacterial operons, and it causes premature termination of transcription.

Summary Introduction

d.

To determine:

The depiction of the given figure if the antisense mechanism was involved in controlling the gene or operon.

Introduction:

The mentioned figure does not provide any evidence that any of the genes or operons are regulated by an antisense transcript.

Summary Introduction

e.

To determine:

The possibility of the transcription of the galM gene from the different promoter.

Introduction:

The given figure depicts galM gene to be a part of the operon containing galETK. There is even a possibility that galM is transcribed from a separate promoter.

Summary Introduction

f.

To determine:

Whether the data shown in the figure reveal the presence of a regulatory mechanism in relation to an sRNA occluding the ribosome binding site.

Introduction:

The ribosome binding site is present in upstream to the start codon. It is a sequence of nucleotides which play an important role in recruiting the ribosome at the initiation of the translation process.

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Students have asked these similar questions
What experimental results would indicate that the mutation lacISlacIS is dominant to lacI+lacI+?   In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a constitutive state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in an activated state in the presence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the presence of lactose.
How long would it take for the E. coli RNA polymerase to synthesize the primary transcript for the E. coli genes encoding the enzymes for lactose metabolism, the 5,300 bp5,300 bp lac operon? Assume an average elongation rate of 7070 nucleotides per second. a)How far along the DNA would the transcription "bubble" formed by RNA polymerase move in 10 seconds10 seconds? b)Assuming that human Pol II transcribes at a similar rate, how long does it take to transcribe the 2,000,000 bp2,000,000 bp dystrophin gene?
1.  a) If glucose is not available and lactose is absent from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?  b)If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?             b)

Chapter 16 Solutions

Genetics: From Genes to Genomes

Ch. 16 - Mutants were isolated in which the constitutive...Ch. 16 - Suppose you have six strains of E. coli. One is...Ch. 16 - The previous problem raises some interesting...Ch. 16 - For each of the E. coli strains containing the lac...Ch. 16 - For each of the following growth conditions, what...Ch. 16 - For each of the following mutant E. coli strains,...Ch. 16 - Maltose utilization in E. coli requires the...Ch. 16 - Seven E. coli mutants were isolated. The activity...Ch. 16 - Cells containing missense mutations in the crp...Ch. 16 - Six strains of E.coli mutants 16 that had one of...Ch. 16 - a. The original constitutive operator mutations in...Ch. 16 - In an effort to determine the location of an...Ch. 16 - Prob. 23PCh. 16 - The footprinting experiment described in Fig....Ch. 16 - Why is the trp attenuation mechanism unique to...Ch. 16 - a. How many ribosomes are required at a minimum...Ch. 16 - The following is a sequence of the leader region...Ch. 16 - For each of the E. coli strains that follow,...Ch. 16 - Prob. 29PCh. 16 - For each element in the list that follows,...Ch. 16 - Among the structurally simplest riboswitches are...Ch. 16 - Great variation exists in the mechanisms by which...Ch. 16 - Many genes whose expression is turned on by DNA...Ch. 16 - In 2005, Frederick Blattner and his colleagues...Ch. 16 - The E.coli MalT protein is a positive regulator of...Ch. 16 - Prob. 36PCh. 16 - Prob. 37PCh. 16 - Prob. 38PCh. 16 - Prob. 39PCh. 16 - Prob. 40PCh. 16 - Prob. 41PCh. 16 - The researchers who investigated bioluminescence...Ch. 16 - Prob. 43PCh. 16 - Quorum sensing controls the expression of...Ch. 16 - Scientists are currently screening a chemical...
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