ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Chapter 17, Problem 26P
Summary Introduction
To determine:
The way by which the antibodies (that bind specifically to DNA fragments containing 5-methylcytosine, but not to DNA lacking modified
Introduction:
The antibody or immunoglobin is a protein which is used by the immune system to neutralize or destroy the pathogen. It is produced by plasma cells of the body. A ChIp seq (ChIP-sequencing) is a technique used to analyze the interaction of proteins.
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Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below.
The resulting phage lysates were then used to infect the recipient strains of S. typhimurium
recipient strains listed in the table.
In each cross, a phenotype was selected for one of the selected for one of the three genetic
markers studied (str, aceA, thrA), and were made to select the recombinants corresponding to the
other two markers. markers.
The results are given in the following table:
Strain
I donor
str thrA
aceA thrA
str aceA+
Strain
recipient
strs thrA+
aceA thrA
str aceA
Phenotype
selected
Str
Ace+
Str
recombinants
selected
ThrA
ThrA
ThrA
ThrA
Ace
Ace
Number
60
40
95
5
10
90
str: gene involved in streptomycin resistance,
aceA: gene involved in the use of acetate as a carbon source,
thrA: gene involved in threonine biosynthesis.
1) What are the selective media used in these three transduction experiments? to obtain the
selected…
A research group is studying a bacterium X that binds to mucosal cells in the lung and invades. Wildtype X has an LD50 value of 10 bacteria when administered to mice by inhalation. Using transposon mutagenesis, the researchers have isolated two mutants of X that they call Xmut1 and Xmut2, both of which have LD50 values of 105 when inhaled by mice. However, in tissue culture cells, Xmut1 can invade the cells just as well as wild-type X, while Xmut2 cannot. Provide a possible explanation for these results.
Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella
typhimurium donor strains described in the table below. The resulting phage lysates were then
used to infect the S. typhimurium recipient strains listed in the table. In each cross, a
phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then
replicates were performed to select the corresponding recombinants for the other two markers.
The results are given in the following table:
Recipient strain Selected phenotype Selected recombinants
Donor
strain
str thrA
aceA+ thrA
str aceA+
strs thrA+
aceA thrA+
str aceA
Str
Ace+
Str
ThrA
ThrA+
ThrA
ThrA+
Ace
Ace
str: gene involved in streptomycin resistance,
aceA gene involved in the use of acetate as a carbon source,
thrA: gene involved in the biosynthesis of threonine.
Number
60
40
95
5
10
90
Determine the order of the genes and draw a genetic map showing this order
Chapter 17 Solutions
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
Ch. 17 - For each of the terms in the left column, choose...Ch. 17 - For each of the following types of gene...Ch. 17 - List five events other than transcription...Ch. 17 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 17 - As shown in the following diagram, a single...Ch. 17 - You have synthesized an enhancerless GFP reporter...Ch. 17 - Prob. 7PCh. 17 - Prob. 8PCh. 17 - A single UAS regulates the expression of three...Ch. 17 - MyoD is a transcriptional activator that turns on...
Ch. 17 - a. Assume that two transcription factors are...Ch. 17 - Prob. 12PCh. 17 - In Problem 12, you identified a genomic region...Ch. 17 - Prob. 14PCh. 17 - Prob. 15PCh. 17 - Genes in both prokaryotes and eukaryotes are...Ch. 17 - Prob. 17PCh. 17 - Lysine 4 of histone H3 H3K4 is methylated in the...Ch. 17 - J.T. Lis and collaborators have developed an...Ch. 17 - Hydatiform moles are growths of undifferentiated...Ch. 17 - Prader-Willi syndrome is caused by a mutation in...Ch. 17 - The human IGF2 gene is autosomal and maternally...Ch. 17 - Follow the expression of a paternally imprinted...Ch. 17 - Reciprocal crosses were performed using two inbred...Ch. 17 - Interestingly, imprinting can be tissue-specific....Ch. 17 - Prob. 26PCh. 17 - A method for detecting methylated CpGs involves...Ch. 17 - Honeybees Apis mellifera provide a striking...Ch. 17 - Consider the experiment in Fig. 17.24, where the...Ch. 17 - A protein or RNA that regulates gene expression in...Ch. 17 - a. How can a single eukaryotic gene give rise to...Ch. 17 - A hunchback gene, a gene necessary for proper...Ch. 17 - You know that the mRNA and protein produced by a...Ch. 17 - You are studying a transgenic mouse strain that...Ch. 17 - Prob. 35PCh. 17 - Scientists have exploited the siRNA pathway to...Ch. 17 - Persimmons Diospyros lotus are dioecious plants,...Ch. 17 - Drosophila females homozygous for loss-of-function...Ch. 17 - The text has discussed the RNA-Seq technique,...Ch. 17 - Researchers know that Fru-M controls male sexual...Ch. 17 - The Drosophila gene Sex lethal Sxl is deserving of...Ch. 17 - Figure 17.29 shows that the Sxl protein binds to...
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