EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 17, Problem 4Q
Summary Introduction
To review:
The level of activity of the enzyme coded by ‘A’ allele of the gene compared to ‘C’ allele.
Given:
Introduction:
The rate of
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For the genotypes and conditions (lactose present or absent) shown in the following Table 2,
predict whether the expression of beta-galactosidase is inducible (I), noninducible (N) or
constitutive (C). Explain your reason.
Table 2
Genotype
I*p*O°z*
Condition
(i)
No lactose
(ii)
I*P*O*Z+
Lactose
I*P-0*Z*
(iii)
(iv)
Lactose
ISP+O°Z+
Lactose
(v)
Ip*o°Z+
No lactose
In the case of normal RNase A, Anfisen found that oxidizing the Cys residues before slowly removing the
urea gave a very different result than oxidizing the Cys after slowly removing the urea.
When the urea was removed first, Anfisen recovered 100% of the catalytic activity. When the urea was
removed after oxidation, only about 1% of the activity (1/105) was recovered. The conclusion was that
104 of every 105 molecules was misfolded, and therefore catalytically inactive.
Let's say someone raises an objection to this interpretation, and says that perhaps, instead, something
chemically happens to the active site of RNase A when it is oxidized before refolding, and that the ~1%
activity measured represents the residual and greatly reduced enzyme activity of all of the molecules,
and the number is just coincidently similar to 1/105. (In other words, instead of 104 completely inactive
molecules for every 1 completely active molecule, all molecules are equally damaged, and only function
at…
For the genotypes and conditions (lactose present or absent) shown in the following Table 2,
predict whether the expression of beta-galactosidase is inducible (I), noninducible (N) or
constitutive (C). Explain your reason.
Table 2
Genotype
I*p*o°z+
Condition
(i)
No lactose
(ii)
(iii)
(iv)
I*P*O*Z+
I*P•O*Z*
I$P*O°Z*
Lactose
Lactose
Lactose
(v)
IP*0°Z+
No lactose
Chapter 17 Solutions
EBK LIFE: THE SCIENCE OF BIOLOGY
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