Concept explainers
(a)
To determine: Whether the given statement about RNA polymerases, “The enzyme is insensitive to alpha-amanitin” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of
(b)
To determine: Whether the given statement about RNA polymerases, “The enzymes catalyze an exergonic reaction” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(c)
To determine: Whether the given statement about RNA polymerases, “All the primary transcripts must be processed before being used in translation” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(d)
To determine: Whether the given statement about RNA polymerases, “The enzymes may sometimes be found attached to an RNA (Ribose nucleic acid) molecule that also has ribosomes bound to it” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(e)
To determine: Whether the given statement about RNA polymerases, “The enzymes synthesize ribosomal RNA” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(f)
To determine: Whether the given statement about RNA polymerases, “Transcription factors must bind to the promoter before the polymerase can bind” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(g)
To determine: Whether the given statement about RNA polymerases, “The enzyme adds a poly (A) sequence to messenger RNA” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(h)
To determine: Whether the given statement about RNA polymerases, “The enzyme moves along the DNA template strand in 3' to 5' direction” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(i)
To determine: Whether the given statement about RNA polymerases, “The enzyme synthesizes a product likely to acquire a 5' cap” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(j)
To determine: Whether the given statement about RNA polymerases, “All promoters used by the enzyme lie mostly upstream of the transcriptional start site and are only partially transcribed” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
(k)
To determine: Whether the given statement about RNA polymerases, “The specificity of transcription by the enzyme is determined by a subunit of the holoenzyme” is true for bacterial, polymerase I, II, or III enzymes.
Introduction: Transcription is a process by which RNA is formed from information stored in the form of nucleotide bases in DNA (Deoxyribose nucleic acid). Three types of RNA polymerase enzymes are used in the case of eukaryotic transcription. These RNA polymerases are RNA polymerase I, II, and III. Only one type of RNA that is known as bacterial RNA polymerase is used in prokaryotic cells.
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Becker's World of the Cell (9th Edition)
- Replication:- what other enzymes are involved in the initiation phase?- explain the role of primers in this phase- how is the building of the leading strand different from that of the lagging strand?arrow_forwardQuestion:- Describe the function of each of the following Shortly. a. Amino-acyl tRNA synthetase b. E coll release factors 1 and 2 (RF1 and RF2) c. 5' methyl-guanosine cap d. Ribosomal P sitearrow_forwardBinding of --------- identifies the decoding center of the ribosome.arrow_forward
- Question:- Fill the Blank! In bacteria, the ___consensus sequence of mRNA binds to the ____rRNA of the 30S small subunit during translation initiation, while in eukaryotes, the _______ consensus sequence of mRNA contains the_____.arrow_forwardIllustrating the importance of triphosphate and monophosphate molecules, explain the process of RNA biosynthesis by RNA polymerase.arrow_forwardSuggest a reasonable strategy for the specific phosphorylation of the5’ –OH group of a nucleoside.arrow_forward
- Calculating human genome If 1.5 percent of the human genome consists of protein-coding sequences, and the entire genome has 3.2x10^9, how many codons are there in the human genome? Remember that a codon is three nucleotides in length.arrow_forwardQuestion:- Arrange the following organisms, in terms of percentage of their genes that are alternatively spliced, from highest to lowest. brewer's yeast streptococcus pyogenes gorillas cricketsarrow_forwardQuestion:- Explain why there are very few sequence-specific DNA-binding proteins that bind to the minor groove of double-stranded DNA.arrow_forward
- How many sites? A researcher has isolated a restriction endonuclease that cleaves at only one particular 10-base-pair site. Would this enzyme be useful in protecting cells from viral infections, given that a typical viral genome is 50,000 base pairs long? Explainarrow_forwardComparing the Mechanisms of Action of EF-Tu/EF-Ts and DnaK/ GrpE (Integrates with Chapter 30.) In what ways are the mechanisms of action of EF-Tu/EF-Ts and Dna K/GrpE similar? What mechanist ic functions do the ribosome A-site and DnaJ have in common?arrow_forwardFunctional Consequences of Y-Family DNA Polymerase Structure The eukaryotic translesion DNA polymerases fall into the Y family of DNA polymerases. Structural studies reveal that their fingers and thumb domains are small and stubby (see Figure 28.10). In addition, Y-family polymerase active sites are more open and less constrained where base pairing leads to selection of a dNTP substrate for the polymerase reaction. Discuss the relevance of these structural differences. Would you expect Y-family polymerases to have 3-exonuclease activity? Explain your answer.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning