Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 18, Problem 2P
Radiotracer Labeling of Pyruvate from Glucose Determine the anticipated location in pyruvate of labeled carbons If glucose molecules labeled (in separate experiments.) with 14C at each position of (.tie carbon skeleton proceed through the glycolytic pathway.
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Calculate the net ATP yield from the complete processing of a saturated fatty acid containing 17 carbons. Consider the β-oxidation steps, processing of acetyl-CoA through the citric acid cycle, and electron transport.
Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0).
I. For one mole of the fatty acid residue, determine the following:
a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC
b. ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue
c. ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residue
Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0).
I. For one mole of the fatty acid residue, determine the following:
a. ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC
b. ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue
c. ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residue
d. net ATP yield of ATP in the complete oxidation of the fatty acid residue
II. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)
Chapter 18 Solutions
Biochemistry
Ch. 18 - Characterizing Glycolysis List the reactions of...Ch. 18 - Radiotracer Labeling of Pyruvate from Glucose...Ch. 18 - Effects of Changing Metabolite Concentrations on...Ch. 18 - Prob. 4PCh. 18 - Prob. 5PCh. 18 - The Reactions and Meehanisms of the Leloir Pathway...Ch. 18 - The Effect of lodoacetic Acid on the...Ch. 18 - Prob. 8PCh. 18 - Comparing Glycolysis Entry Points for Sucrose...Ch. 18 - Prob. 10P
Ch. 18 - Prob. 11PCh. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - Energetic of Fructose-1 ,6-bis P Hydrolysis...Ch. 18 - Prob. 15PCh. 18 - Energetics of the Hexokinase Reaction The...Ch. 18 - Prob. 17PCh. 18 - Distinguishing the Mechanisms of Class I and Class...Ch. 18 - Prob. 19PCh. 18 - Understanding the Mechanism of Hemolytic Anemia...Ch. 18 - Prob. 21PCh. 18 - Based on your residing of this chapter, what would...Ch. 18 - Examine the ActiveModel for alcohol dehydrogenase...Ch. 18 - Based on your knowledge of the structure of NAD+...Ch. 18 - Using the ActiveModel for phosphofructokinase...
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- Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0). II. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)arrow_forwardThe large amount of energy used during aerobic exercise(e.g., running) requires large amounts of oxaloacetate.Explain why acetyl-CoA cannot be used to produceoxaloacetate in this circumstance. What is the sourceof oxaloacetate molecules during aerobic activity?arrow_forwardAlthough phosphoesters are generally not energetic enough to donate the phosphate to make a high energy bond (e.g., a phosphoanhydride), the phosphate in PEP is used to convert ADP to ATP in the pyruvate kinase reaction. Carefully explain the ‘quirk’ of this reaction that makes it energetically feasible.arrow_forward
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- Consider the complete oxidation of one mole of simple TAG containing behenic acid residues (22:0). I. For one mole of the fatty acid residue, determine the following: a. What is the number of ATP yield obtained from the oxidation of acetyl CoA entering the TCA cycle and ETC b. What is the number of ATP yield obtained from NADH coming from the complete β-oxidation of the fatty acid residue c. What is the number of ATP yield obtained from FADH2 coming from the complete β-oxidation of the fatty acid residue d. What is the number of net ATP yield of ATP in the complete oxidation of the fatty acid residue II. What is the net ATP yield for the complete oxidation of all the fatty acid residues of the simple TAG? (Note: glycerol backbone is not included)arrow_forwardOf the steps illustrated how many are unidirectional? Identify the substrate and product of each of these steps. Identify the three enzymes that are likely to be regulated in glycolysis? How did you make this determination. Glycogen synthase is an enzyme that catalyzes the synthesis of glycogen from glucose. Glucose 6 phosphate activates glycogen synthase by allosteric regulation. Illustrate and describe how this allosteric modulation is likely to occur? Glycogen phosphorylase, an enzyme that catalyzes the breakdown of glycogen to form glucose, is active when a phosphate group is added. This is an example of enzyme regulation by ____________________________.arrow_forwardYou are subsequently instructed to improve the biochemical function of pyruvate kinase,which aids in the conversion of phosphoenolpyruvate to pyruvate. Suggest an approach thatcan be conducted to achieve this goal. Compare the approach that you have chosen withother existing procedures, such as construction of mutant enzymes.arrow_forward
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