Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Textbook Question
Chapter 18, Problem 5P
Consider the even
a. How are the sharp boundaries of expression of eve stripe
b. Consider the binding sites for gap proteins and Bicoid in the stripe
c. Explain what you expect to see happen to even-skipped stripe
Figure 18.9
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
a. The eyeless gene is required for eye formation in Drosophila. It encodes a homeodomain. What would you predict about the biochemical function of the Eyeless protein?b. Where would you predict that the eyeless gene is expressed in development? How would you test your prediction? c. The Small eye and Aniridia genes of mice and humans, respectively, encode proteins with very strong sequence similarity to the fly Eyeless protein, and they are named for their effects on eye development. Devise one test to examine whether the mouse and human genes are functionally equivalent to the fly eyeless gene.
Describe the common signal transduction event that is perturbed by cancer-promoting mutations in the genes encoding RAS and NF-1. Why are mutations in RAS more commonly found in cancers than mutations in NF-1?
Using RNA-seq analysis of transcripts isolated from different organs in mice, you find that the GFAPgene is expressed only in the brain. You examine expression in glia and neurons (two different celltypes found in the brain) and determine that GFAP mRNA and protein is made only in glia. Thisgene has never been studied before, so you start by characterizing the cis-acting elements requiredfor brain-specific expression.
a. You decide to fuse pieces of DNA found upstream regions of the coding region of GAP to areporter gene (lacZ) and monitor expression. Different fragments (shown as dark lines) werecloned next to the lac gene that lacked its own promoter. M and H are restriction enzymesites. From the data shown, determine which region contains the promoter and whichregion contains an enhancer . Explain.
Chapter 18 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 18 - 18.1 Explain why many developmental genes encode...Ch. 18 - Bird beaks develop from an embryonic group of...Ch. 18 - 18.3 How is positional information provided along...Ch. 18 - Early development in Drosophila is atypical in...Ch. 18 - 18.5 Consider the evenskipped regulatory sequences...Ch. 18 - What is the difference between a parasegment and...Ch. 18 - Why do loss-of-function mutations in Hox genes...Ch. 18 - 18.8 Compare and contrast the specification of...Ch. 18 - Prob. 9PCh. 18 - Ablation of the anchor cell in wild type C....
Ch. 18 - 18.11 In gain-of-function and. elegans mutants,...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - 18.14 Given that maternal Bicoid activates the...Ch. 18 - What phenotypes do you expect in flies homozygous...Ch. 18 - The pair rule gene fushitarazu is expressed in...Ch. 18 - 18.17 In contrast to Drosophila, some insects...Ch. 18 - Prob. 18PCh. 18 - 18.19 You are traveling in the Netherlands and...Ch. 18 - 19.20 A powerful approach to identifying genes of...Ch. 18 - The Hoxd 913 genes are thought to specify digit...Ch. 18 - Three-spined stickleback fish live in lakes formed...Ch. 18 - The flowering jungle plant Lacandoniaschismatica,...Ch. 18 - 18.24 Homeotic genes are thought to regulate each...Ch. 18 - Prob. 25PCh. 18 - Basidiomycota is a monophyletic group of fungi...Ch. 18 - Prob. 27PCh. 18 - In C. elegans there are two sexes: hermaphrodite...Ch. 18 - In Drosophila, recessive mutations in the...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- . Another class of suppressor mutations, not describedin the chapter, are mutations that suppress missensemutations.a. Why would bacterial strains carrying such missense suppressor mutations generally grow moreslowly than strains carrying nonsense suppressormutations?b. What other kinds of mutations can you imagine ingenes encoding components needed for gene expression that would suppress a missense mutationin a protein-coding gene?arrow_forward(a) Did deletion of any of the possible control elements cause areduction in reporter gene expression? If so, which one(s), and howcan you tell? (b) If loss of a control element causes a reduction ingene expression, what must be the normal role of that controlelement? Provide a biological explanation for how the loss of sucha control element could lead to a reduction in gene expression.arrow_forwardGene X is expressed in the developing brain, heart, andlungs of mice. Mutations that selectively affect gene Xfunction in these three tissues map to three differentregions (A, B, and C, respectively) 5′ of the X codingregion.a. Explain the nature of these mutations.b. Draw a map of the X locus consistent with the preceding information.c. How would you test the function of the A, B, and Cregions?arrow_forward
- This data shows the efficacy of different treatments on the survival rates of patients with glioblastomas, as well as methylated, and on methylated MGMT promoters. A. Explain the trends in the figure B. Relate MGMT methylation to the central dogma/flow of genetic information, and how genes are expressed. arrow_forwardExplain why loss-of-function hedgehog and smoothened mutations yield the same phenotype in flies, but a loss-of- function patched mutation yields the opposite phenotype.arrow_forwardThe UG4 gene is expressed in the stem and leaf tissue of the plant Arabidopsis thaliana. To identify potential mechanisms regulating UG4 gene expression, six small deletion mutations are made in cloned sequence containing the upstream regulatory region. The full length mutant segments Which mutation(s) affect an enhancer? Why? Which mutations identify the promoter? Why? Speculate about the reason for different transcription rated obtained for fusion constructs E and F.arrow_forward
- The gene Igf2 for the insulin-like growth factor (IGF) promotes growth hormone production and cell proliferation, and is maternally imprited. The IGF receptor gene Igf2r counters the effects of the Igf2 gene and is paternally imprinted. In knockout experiments with mice, which combination of manipulated genes will result in mice that will grow to normal size? (a) The maternally derived Igf2r and paternally derived igf2 are both normally expressed. (b) The maternally derived Igf2r and paternally derived igf2 are both deleted. (c) The maternally derived Igf2r is normally expressed but the paternally derived Igf2 is deleted. (d) The paternally derived Igf2 is normally expressed but the maternally derived Igf2r is deleted. A. (c) and (d) B. (d) only C. (a) and (b) D. (a) onlyarrow_forwardDiscuss the method and experiments where Simplifying Protein Expression with myTXTL is usedarrow_forwardUsing coat color in mice and the development of female honeybees as examples, explain how dietary factors cause epigenetic modifications and thereby lead to phenotypic effects.arrow_forward
- For the Igf2 gene, where do de novo methylation and maintenancemethylation occur?a. De novo methylation occurs in sperm, and maintenancemethylation occurs in egg cells.b. De novo methylation occurs in egg cells, and maintenancemethylation occurs in sperm cells.c. De novo methylation occurs in sperm, and maintenancemethylation occurs in somatic cells of offspring.d. De novo methylation occurs in egg cells, and maintenancemethylation occurs in somatic cells of offspring.arrow_forwardWhat is the advantage and disadvantage of gene repression in development and What does it mean when we say "enhancers work in a combinatorial fashion?arrow_forwardExplain the signaling mechanism of retinoic acid, the source of retinoic acid in vivo, and how retinoic acid is important for the anteroposterior pattern of Hox gene expression?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Embryology | Fertilization, Cleavage, Blastulation; Author: Ninja Nerd;https://www.youtube.com/watch?v=8-KF0rnhKTU;License: Standard YouTube License, CC-BY