Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 6P
This problem concerns a technique called enhancer trapping which scientists first developed in Drosophila. The purpose is to find enhancers in the genome and thereby to identify genes that are active in particular cell types. In enhancer trapping, thousands of fly lines are created, each of which has a single copy of the transgene shown here integrated into a random location in the genome via P element–mediated gene transfer. Note that the transgene has a promoter but no enhancer. (The thick horizontal arrows indicate the inverted repeats at the ends of P elements.)
| a. | How could you identify fly lines in which the transgene integrated next to an enhancer? |
| b. | Describe how you could use this technique to identify genes expressed specifically in the fly wing. |
| c. | Do you think that homozygotes for any of the transgene insertions might have a mutant |
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Chapter 18 Solutions
Genetics: From Genes to Genomes
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...
Ch. 18 - The goal of the Knockout Mouse Project is to...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - a. Which genome manipulation technique would you...Ch. 18 - a. Diagram a knockin construct that could have...Ch. 18 - Prob. 16PCh. 18 - Prob. 17PCh. 18 - The transcription factor Pax6 is required...Ch. 18 - Mouse models for human genetic diseases are...Ch. 18 - One way to determine where inside a cell a protein...Ch. 18 - In Problem 5 in Chapter 17, you saw that a SNP...Ch. 18 - Scientists now routinely use CRISPR/Cas9 to make...Ch. 18 - Geneticists are currently considering using...Ch. 18 - a. Figures 18.9 and 18.12 demonstrated methods to...Ch. 18 - Nonhomologous end-joining NHEJ of a double-strand...Ch. 18 - One problem that researchers sometimes encounter...Ch. 18 - Researchers at the University of California at San...Ch. 18 - Prob. 28PCh. 18 - F. Port and S. Bullock at the University of...Ch. 18 - On Fig 18.14, locate the PAM site and identify the...Ch. 18 - Prob. 31PCh. 18 - Prob. 32PCh. 18 - Recall that Leber congenital amaurosis LCA, a form...Ch. 18 - One potential strategy for gene therapy to correct...Ch. 18 - Recently, scientists have used a mouse model for...
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- Let's say: a drosophila melanogaster line is bred in a lab, which has a phenotypic variation of interest resulting from the wingless gene (wg1) . Firstyly: how you would go about identifying SNP variants in this gene? and Secondly: what method could you use to eliminate the possibility that this phenotype results from other gene regulatory elements?arrow_forwardHow do I draw the sequence and explain the process for each step? Draw the sequence of molecular events that occurs to induce STAT transcription factor localization to the nucleus. To complete this, you will draw the first step that occurs, then draw a new figure with the second step that occurs, then draw a new figure with the third step that occurs, and so on until you have completed all of the steps. On the drawing, briefly label each molecular event (each drawing). For this brief label, explain what is happening during each step.arrow_forwardExpression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use. In an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. Will this strategy result in good expression of gene X? Why or why not? please try to explain a bit elaborately.arrow_forward
- Geneticists often use ethylmethane sulfonate (EMS) to induce mutations in Drosophila. Why is EMS a mutagen of choice for genetic research? What would be the effects of EMS in a strain of Drosophila lacking functional mismatch repair systems?arrow_forwardMice usually have wild-type agouti fur that appears grey, but a mouse geneticist has a pure-breeding white-furred strain that is homozygous for a recessive mutation. Molecular analysis shows that the mutation is a missense mutation in an exon common to three alternatively spliced forms of a gene expressed in hair follicles. Which of the following transgenic animals would be most useful to determine which spliced form (or forms) is sufficient to specify agouti fur?A. Construct a GFP reporter using the promoter for the identified gene. B. Express a wild-type version of a cDNA for each spliced form in the white mutant mouse to see which form(s) rescue the phenotype. C. Use CRISPR/Cas9 to make a large deletion of the entire coding region of the identified gene. D. Introduce the wild-type genomic allele into the mutant mouse to test for rescue of the mutant phenotypearrow_forwardWhen a region of DNA that contains the genetic information for a protein is isolated from a bacterial cell and inserted into a eukaryotic cell in a proper position between a promoter and a terminator, the resulting cell usually produces the correct protein. But when the experiment is done in the reverse direction (eukaryotic DNA into a bacterial cell), the correct protein is often not produced. Can you suggest an explanation?arrow_forward
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