The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment. Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Question

Want to see the full answer?

Answer 1

Question

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Q21: Polymerase Chain Reaction (PCR) (Links to an external site.) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus (Links to an external site.) to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.2 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 2.3 nanograms per microliter? Report your answer to two decimal places using standard decimal notation (e.g. 0.5, not 5.0e-1)

In bacterial transformation ... 1, Why do you have to incubate the competent bacterial cells on ice after the addition of plasmid DNA? 2. Why do you have to add SOC (Super Optimal Broth with Catabolite repression) medium to bacterial cells? 3. Why do you have to incubate the bacterial cells in SOC medium at 37oC for 45 minutes?

Question:- How many different types of alterations in chromosomal numbers are there? -How can I use the F plasmid to introduce the transfer of the URA+ gene from a wild type strain into the ura- mutant strain you isolated in Q5. -After obtaining the ura- mutant, I want to transform this ura- mutant back to wild type with a DNA sample prepared from the wild type cell. How can I achieve this transformation experimentally?

Answer 2

Question

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Answer 6

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Answer 7

Chapter 19.5, Problem 2EQ

Summary Introduction

To determine: The reason for no growth of the unmixed bacterial strains in the Lederberg and Tatum experiment.

Introduction: The experiment performed by Lederberg and Tatum was conducted to understand the mechanism of transformation in bacteria. The experiment was later continued by Davis to finally prove that direct contact is pre-requisite for conjugation to occur.

Answer 8

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Answer 9

Summary Introduction

To determine: The significance of the mixed strains on the culture plate.

Introduction: The mixing of the strains met– bio– thr+ pro+ and met+ bio+ thr– pro– did not produce colonies due to lack of the mechanisms to synthesize the required amino acids. However, the mixing of the strains lead to production of new strain, which was capable of growing on the culture media

Answer 10

Expert Solution & Answer

Answer 11

Expert Solution & Answer

Answer 12

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 13

Ch. 19.1 - Prob. 1CS Ch. 19.2 - Prob. 1CS Ch. 19.2 - Viral Reproductive Cycles Concept Check: From the...Ch. 19.4 - Genetic Properties of Bacteria Core Skill:...Ch. 19.4 - Prob. 1CC Ch. 19.4 - Genetic Properties of Bacteria Concept Check:...Ch. 19.4 - Prob. 3CC Ch. 19.5 - Prob. 1CC Ch. 19.5 - Prob. 1EQ Ch. 19.5 - Prob. 2EQ

Solution Summary: The author explains Lederberg and Tatum's experiment to understand the mechanism of transformation in bacteria, and Davis' experiments to prove that direct contact is pre-requisite for conjugation.

Concept explainers

Videos

Want to see the full answer?

Chapter 19 Solutions