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Concept explainers
(a)
Interpretation: The validation of the substance, heat as a denaturing agent for protein has to be stated.
Concept introduction: The disruption of secondary, tertiary and quaternary structural interactions in a protein results in the partial or complete disorganization of its three-dimensional shape of proteins. This process is known as protein denaturation. The process of denaturation of proteins takes place in the presence of denaturing agents.
(b)
Interpretation: The validation of the substance, ultraviolet radiation as a denaturing agent for protein has to be stated.
Concept introduction: The disruption of secondary, tertiary and quaternary structural interactions in a protein results in the partial or complete disorganization of its three-dimensional shape of proteins. This process is known as protein denaturation. The process of denaturation of proteins takes place in the presence of denaturing agents.
(c)
Interpretation: The validation of the substance, ethanol as a denaturing agent for protein has to be stated.
Concept introduction: The disruption of secondary, tertiary and quaternary structural interactions in a protein results in the partial or complete disorganization of its three-dimensional shape of proteins. This process is known as protein denaturation. The process of denaturation of proteins takes place in the presence of denaturing agents.
(d)
Interpretation: The validation of the substance, strong base as a denaturing agent for protein has to be stated.
Concept introduction: The disruption of secondary, tertiary and quaternary structural interactions in a protein results in the partial or complete disorganization of its three-dimensional shape of proteins. This process is known as protein denaturation. The process of denaturation of proteins takes place in the presence of denaturing agents.
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Chapter 20 Solutions
EBK GENERAL, ORGANIC, AND BIOLOGICAL CH
- Describe step by step process of: a. Ion-exchange Chromatography b. Hydrophobic Interaction Chromatography c. Affinity Chromatography d. Reversed-Phase Chromatography e. Principle of Polyacrylamide Gel Electrophoresis f. Isoelectric Focusing g. Protein Sequencingarrow_forwardIdentify the base and sugar in each of the following nucleotides: A. CMP B. dAMP C. dTMP D. UMParrow_forwarda) Describe the basis of affinity chromatography in protein purification.c. What is the most appropriate method of protein elution in affinity chromatography?d. List three examples of commonly employed combinations of ligand and protein in affinitychromatography.arrow_forward
- Define the following terms:a. alkylating agentb. base analoguec. nonalkylating agentd. intercalating agente. ethidium bromidearrow_forwardName another condition besides heat and exposure to a bond disruptor (like alcohol) that could affect the ability of a protein to maintain its shape.arrow_forwardMass spectrometry and X‑ray diffraction are common biochemical techniques for characterizing proteins. Classify each statement based on whether it applies to mass spectrometry, X‑ray diffraction, or both techniques.arrow_forward
- Define the following terms: a. β-hairpin b. supersecondary structure c. fibrous protein d. globular protein e. mosaic proteinarrow_forwardFive amino acids were subjected to normal phase chromatography, and the following Rf values were computed: A - 0.59 B - 0.12 C - 0.71 D - 0.33 E - 0.20 Which is the most polar amino acid? Group of answer choices C B E A Darrow_forwardIdentify the biomolecules being described. (Proteins, nucleic acid, carbohydrates, lipids). A. It is made up of two patty acids, an alcohol, and a phosphate group. B. It is composed of amino acid.arrow_forward
- Five amino acids were subjected to reverse phase chromatography, and the following Rf values were computed: A - 0.23 B - 0.08 C - 0.54 D - 0.74 E - 0.96 Which is the most polar amino acid? Group of answer choices E D B C Aarrow_forwardBinding additives are polysaccharides that have functions of: A. Encapsulating and aerating B. Anti-splash and stabilizer C. Thickeners and stabilizers d. Clouding and Hydrolyzingarrow_forwardWhich of the following will happen if perchloric acid is added to a neutral polysaccharide gel? (Select 2 Possible Answers) a. increases gel formation b. decreases gel formation c. increases sol formation d. decreases sol formationarrow_forward