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Interpretation:
The weight of ATP in the given original sample has to be calculated.
Concept Introduction:
Beer-Lambert’s law:
It is the law that is widely used to make quantitative analysis of samples in UV-Spectroscopy. This law establishes a relation between absorbance, concentration and path-length of the sample cell which is known as cuvette in UV-Spectroscopy. The expression of the law is shown here:
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Chapter 20 Solutions
Student Study Guide and Solutions Manual for Brown/Iverson/Anslyn/Foote's Organic Chemistry, 8th Edition
- A specific steroid has a λmax of 249 nm and a molar absorptivity ε of 11,500 L mol−1 cm–1. What is the concentration of the compound in a solution whose absorbance at 249 nm is A = 0.050 with a sample pathlength of 1.00 cm?arrow_forward50 uL of an aqueous sample of double-stranded DNA is dissolved in 950uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What isthe concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wandering as well if the total diluted volume would be 950ul + 50ul = 2000ularrow_forwardA sample of adenosine triphosphate (ATP) (MW 507, e 5 14,700 M-1cm-1 at 257 nm) is dissolved in 5.0 mL of buffer. A 250-mL aliquot is removed and placed in a 1 cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 1.15. Calculate the weight of ATP in the original 5.0 mL sample.arrow_forward
- In an analytical laboratory a chemical compound namely Paracetamol (151.163 g/mol) is determined in a sample. A sample weighing 0.0295 g was dissolved in a solvent and diluted the solution to 1 L. The solution has λ max at 243 nm (ε=2.6×104 cm-1 mol-1 L). The solution exhibits an absorbance of 0.638 in a 2 cm cell. Calculate the percentage paracetamol in the sample.arrow_forwardAbsorbance at 453 nm 8393939 7 fl. oz./20 ml Beer-Lambert's Law (Spectrophotometry) #1 Fe (aq) + KSCN(s) FESCN "(aq) + K" (aq) 3+ To determine the moles of Fe (aq) in a 100. mL sample of an unknown solution, excess KSCN(s) is added to convert all the Fe (aq) into the dark red species FeSCN"(aq), as represented by the equation above. The absorbance of FESCN"(aq) at different concentrations is shown in the graph below. 2+ 0.50 目0.40 0.30 0.20 01 0. 0. 5 x 10-5 10 x 10-5 Concentration of FESCN2+ (M) If the absorbance of the mixture is 0.20 at 453 nm, haw many moles of Fe (aq) were present in the 100. mL sample? (Assume that any volume change due to adding the KSCN(s) Iis negligible.) 3+, 4x10-4 mol 3 x 104 mol (B) 4x 10-6 mol 3x 10-6 molarrow_forwardA student wanted to determine the level of lead in amoxicillin powder, so he transferred 148 mg of the powder to 200 ml volumetric flask and completed the volume volumetrically, then he transferred 5 ml of the obtained solution to 50 ml volumetric flask and completed the volume properly, and read the absorbance of the obtained solution on AAS. The reading was 0.48. and the calibration equation was: "Y =12.25 x- 0.05" (concentration microg./mL). ?"Calculate the content of lead in amoxicillin as "ppm 326.5.a O 605.0 .b O 60.5.c O 17.5.d O 584.7.e O أخل اختياريarrow_forward
- A student wanted to determine the level of lead in amoxicillin powder, so he transferred 148 mg of the powder to 200 ml volumetric flask and completed the volume volumetrically, then he transferred 5 ml of the obtained solution to 50 ml volumetric flask and completed the volume properly, and read the absorbance of the obtained solution on AAS. The reading was 0.48. and the calibration equation was: "Y =12.25 x- 0.05" (concentration microg./mL). Calculate the content of lead in amoxicillin as "ppm"? O a. 326.5 O b. 605.0arrow_forwardThe standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate solutions of known concentration. You are asked to analyze a Fe(SCN)2+ solution with an unknown concentration and an absorbance value of 0.392. The slope-intercept form of the equation of the line is y = 4538.1x +0.0077. The unknown was analyzed on the same instrument as the standard curve solutions at the same temperature. What is the Fe³+ concentration of the unknown solution? [Fe³+] = mol/L Absorbance Iron(III) thiocynate standard curve 1.0 V 0.5 0.0001 Fe³+ concentration (M) 0 0.0002arrow_forwardThe intensity of light passing through a 1.00-cm sample of a solution of polystyrene in methyl ethyl ketone was observed to decrease by O.9%. The concentration of the solution was 10.6 kgm-3. Given A =546.1 nm, no= 1.377, and dn/dC" = 2.20x 10-4 %3D m3/ kg. calculate the molar mass of the polystyrene in kg/mol. The relative displacement (r/ro) of bovine serum albumin was observed as a function of time: t (s) 700 3580 4540 5020 r/r. 1.0129 1.0679 1.0871 1.0965 6 260 /s, find the sedimentation coefficient. Assuming Given w = v = 7.34x 10 3 m3 kg and p = 9.93 x 102 kg/m3, diffusivity = 6.97 x 1011 m2/s at 25 °C, determine the molar %3D mass of the sample in kg/mol.arrow_forward
- You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).arrow_forwardOxybenzone is used in sunscreens. A UV spectrum of a 2.0 mL sample of oxybenzone (228.2 g/mol) in a 1.0 cm cell was obtained. The molar extinction coefficient for oxybenzone is 14315 M⁻¹cm⁻¹. The UV spectrum revealed a λ(max) of 295 nm with an absorbance of 0.85. Calculate the mass of oxybenzone in the solution.arrow_forwardIn a pharmaceutical industry a drug Paracetamol (151.163 g/mol) is determined in a sample. A 0.0315 g samples was reacted with methanol and diluted the solution to 1 L. the solution λ max at 243 nm (ε=2.6×104 cm-1 mol-1 L). The solution exhibits an absorbance of 0.642 in a 2 cm cell. Calculate the percentage paracetamol in the sample.arrow_forward
- Organic ChemistryChemistryISBN:9781305580350Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. FootePublisher:Cengage LearningMacroscale and Microscale Organic ExperimentsChemistryISBN:9781305577190Author:Kenneth L. Williamson, Katherine M. MastersPublisher:Brooks Cole
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