Campbell Biology with MasteringBiology Canvas Direct Integration for BSC 2011 University of South Florida, 1/e
1st Edition
ISBN: 9781323442982
Author: Reece
Publisher: Pearson Education
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Chapter 20.1, Problem 4CC
VISUAL SKILLS Ø Compare Figure 20.7 with Figure 16.20. How does replication of DNA ends during PCR proceed without shortening the fragments each time?
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VISUAL SKILLS If the DNA pol I in a given cell werenonfunctional, how would that affect the synthesis ofa leading strand? In the overview box in Figure 16.17,point out where DNA pol I would normally function onthe top leading strand.
PCR Review:
How are you able to target specific genes in PCR?
What were some possible sources of error during your DNA Extraction and PCR experiments?
Transcribe and translate the DNA strand Remember to use the start and stop sequences.
ACGGTACCGTTAGCCGACATCGGGGACACTGACTCG
Chapter 20 Solutions
Campbell Biology with MasteringBiology Canvas Direct Integration for BSC 2011 University of South Florida, 1/e
Ch. 20.1 - Prob. 1CCCh. 20.1 - DRAW IT One Strand of a DNA molecule has the...Ch. 20.1 - What are some potential difficulties in using...Ch. 20.1 - VISUAL SKILLS Compare Figure 20.7 with Figure...Ch. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - DNA technology has many medical applications....Ch. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 9TYUCh. 20 - MAKE CONNECTIONS Looking at Figure 20.15, what...Ch. 20 - DRAW IT You are cloning an aardvark gene, using a...Ch. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 13TYUCh. 20 - Prob. 14TYUCh. 20 - The water in the Yellowstone National Park hot...
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- Which technique rapidly replicated specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reactionarrow_forwardWhat 4 steps would you need to do to extract DNA?arrow_forwardTOPIC: PCR and Gene Cloning Basics Question: What are 2 possible roles of CaCl2 in the transformation process?arrow_forward
- Please do it fast What gene sequences are selected during DNA barcoding?.arrow_forwardCompare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the same gene cloned by reverse transcriptase PCR (RTPCR).arrow_forwardDuring proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicasearrow_forward
- DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to each of the experimental tubes. What would happen if you underloaded the enzyme? i.e. you only delivered 3 or 4 μl ? What would see in your gel results?arrow_forwardCorrect order ib which the following enzynes would operate to fix a damaged nucleotide in a human gene. a) nuclease, DNA polymerase, RNA primase b) helicase, DNA polymerase, DNA ligase c) DNA ligase, nuclease, helicase d) nuclease, DNA polymerase, DNA ligasearrow_forwardTranscribe and translate the DNA strand below. Remember to use the start and stop sequences. UGCGAUGGCAAUCGGCUGUAGCCCCUGUGACUGAGCarrow_forward
- DNA: How PCR works and why it's useful?arrow_forwardPlease help with sub questions 1a 1b and 1c DNA: 1a. Whats the difference between continuous and discontinuous synthesis? 1b. Why replication requires both? 1c. How was this determined or How was this discovered?arrow_forwardshow your rationale How many RT-PCR products generated from 13 copies of mRNA (= cDNA) from 33 PCR amplification cycles? What was the original starting number of mRNA molecules if after 36 PCR cycles, the reaction had generated 996,432,412,672 copies?arrow_forward
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