Campbell Biology with MasteringBiology Canvas Direct Integration for BSC 2011 University of South Florida, 1/e
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Chapter 20.1, Problem 1CC
Summary Introduction

To determine: The bonds cleaved when restriction enzyme PvuI cuts its specific site in the DNA sequence.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.

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MAKE CONNECTIONS Although the proteins that cause theE. coli chromosome to coil are not histones, what propertywould you expect them to share with histones, given theirability to bind to DNA (see Figure 5.14)?
8. Altering Linking Number (a) What is the Lk of a 5,000 op circular duplex DNA molecule with a nick in one strand? (b) What is the Lk of the molecule in (a) when the nick is sealed (relaxed)? (c) How would the Lk of the molecule in (b) be affected by the action of a single molecule of E. coli topoi somerase 12 (d) What is the Lk of the molecule in (b) after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP? (e) What is the Lk of the molecule in (d) after four enzymatic turnovers by a single molecule of bac terial type I topoisomerase? (1) What is the Lk of the molecule in (d) after binding of one nucleosome core?
RNA polymerase from E. coli (core enzyme alone) has all of the following properties except: a)requires all four ribonucleoside triphosphates and a DNA template. b)can extend an RNA chain and initiate a new chain. c)recognizes specific start signals in DNA. d)produces an RNA polymer that begins with a 5'-triphosphate. e)is required for the synthesis of mRNA, rRNA, and tRNA in E. coli.

Chapter 20 Solutions

Campbell Biology with MasteringBiology Canvas Direct Integration for BSC 2011 University of South Florida, 1/e

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