Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 21, Problem 6TY
In the CRISPR-Cas technology for editing genes, what is (are) the function(s) of sgRNA?
- a. to bind to the target gene
- b. to bind to Cas9
- c. to cause a double-strand break in the target gene
- d. all of the above
- e. both a and b
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Students have asked these similar questions
The CRISPR-Cas9 system can be used to:
a.
sequence a genome in living cells
b.
edit a gene in living cells and organisms
c.
diagnose sickle-cell anemia
d.
identify a specific gene
e.
detect gene expression under different conditions
Which of the following can be a achieved by CRISPR/Cas9-based genome editing tool?
A. Knocking out one or more gene
B. Activating expression of a gene
C. Suppressing expression of a gene
D. All of the above
We have isolated a mutant that changes transcription of many mRNAs, we can use to determine if there are changes to mRNA levels
A.
microarrays/Sequencing technologies
B.
Sanger sequencing
C.
western blot
D.
all of the above
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- If you want to express a eukaryotic gene in a bacterial cell, you ned to (select all that apply): a.) add a bacterial promoter in front of the gene b.) remove introns from the gene c.) add a polyA tail sequence d.) add eukaryotic tRNAsarrow_forwardRNA ploymerase binds a. to the coding region of the gene b.to the 3 prime end of the gene c.to the 5 prime end of the gene d.to the promoter region at the upstream region of the gene e.none of the above answers is correctarrow_forwardQ. Which of the following statements about CRISPR-Cas9 is correct? A. It relies on the natural process of DNA repair. B. Regardless of the DNA target, the sequence of the guide RNA is always the same. C. Cas9 is only involved in recruitment of guide RNA to its target DNA. D. It is not precise enough to make a single base pair edit.arrow_forward
- Which of the following is NOT a true statement about CRISPR/Cas9?A. CRISPR/Cas9 is simpler to design than other gene editing technologies.B. CRISPR/Cas9 is derived from a bacterial adaptive immune defense system.C. CRISPR/Cas9 is more efficient than other gene editing technologies.D. CRISPR/Cas9 uses an RNA guide to edit a gene.E. Delivery of CRISPR/Cas9 is easier than delivery of other gene editing technologies.arrow_forwardCRISPR is a powerful gene editing tool because a. the programmable enzyme (Cas9) can find an exact sequence and make a cut at precise nucleotide. b. it can be applied to all types of cells. c. it has both genetic editing and epigenetic functions. d. it is inexpensive. e. all of these are correctarrow_forwardESTs are ________. a. generated after a cDNA library is made b. unique sequences in the genome c. useful for mapping using sequence information d. all of the abovearrow_forward
- Part A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forwardWhat will most likely happen when the -35 element and pribnow box are mutated? a. The sigma factor will not be able to bind to TBP. b. The rate of transcription will increase. c. RNA Polymerase II will not be able to bind to the promoter region. d.arrow_forwardexplain what CRISPR/cas13 is and how is it different from CRISPR/cas9, the gene editing toolsarrow_forward
- The purpose of a ChIP-chip assay is to determinea. the expression levels of particular genes in the genome.b. the sites in a genome where a particular protein binds.c. the amount of a specific protein that is made in a given cell type.d. any of the above.arrow_forwardA collection of recombinant vectors that carry fragments of chromosomalDNA is calleda. a genomic library.b. a cDNA library.c. a Northern blot.d. either a or b.arrow_forwardThe ab initio approach finds genes by looking for a. common sequences found in most genes. b. similarity in sequence with known genes. c. mRNA with the use of in situ hybridization. d. mutant phenotypesarrow_forward
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