Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 21.1, Problem 1CS
Summary Introduction
To explain: The general properties of the plasmid.
Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of both the coding as well as the non-coding parts of DNA. The study of the genome is known as genomics. Genomics contains an important technique by which multiple and exact copies of a gene can be produced. This technique is known as gene cloning.
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2. If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type of medium will be used? How will it work?
VISUAL SKILLS Compare Figure 20.7 with Figure 16.20.How does replication of DNA ends during PCR proceedwithout shortening the fragments each time?
Expand PCR? Describe the different Steps involved in this technique?
Chapter 21 Solutions
Biology
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- Compare the possible differences between a eukaryotic protein-encoding gene cloned by PCR and the same gene cloned by reverse transcriptase PCR (RTPCR).arrow_forwardVISUAL SKILLS Consider the microarray in Figure 20.12.If a sample from normal tissue is labeled with a green fluorescent dye and a sample from cancerous tissue is labeledred, what color spots would represent genes you would beinterested in if you were studying cancer? Explain.arrow_forwardDescribe how PCR is used to amplify a specific gene from total cellular DNA.arrow_forward
- Describe at least one important application of DNA technology in each of the following fields: medicine, DNA fingerprinting, and transgenic organisms.arrow_forwardPreforming a "blue-white screen" 3) Would bacteria that have taken up a plasmid into which a DNA fragment has been inserted, form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.arrow_forwardTOPIC: PCR and Gene Cloning Basics Question: What are 2 possible roles of CaCl2 in the transformation process?arrow_forward
- PCR Review: How are you able to target specific genes in PCR? What were some possible sources of error during your DNA Extraction and PCR experiments?arrow_forwardQ. Based on your understanding of plasmids’ answer the following questions.1. Why is a daughter cell that fails to inherit a kind of plasmid dies? 2. How transfer of Col E1 plasmid to an F cell occurs? 3. What component you will must include in constructing a plasmid cloning vector that can replicate in both bacteria and yeast? 4. How copy number of a plasmid may affect its segregation? 5. How the yeast 2u circle maintains 80 copies in a cell?arrow_forwardWhat type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology experiments? Identify those two enzymes used to cut and paste both genes into the plasmid. Identify all three strategies used in this lab to maximize transformation success. Explain what it means for bacteria to be “competent.” Explains why bacterial competency is this important for this investigation.arrow_forward
- Differentiate between plasmids, phage-based cloning vectors, cosmids, and artificial chromosomes in terms of structure and applicationarrow_forwardQ11) Explain, using the pCR 2.1 vector, what we would see if we try to grow cells on a plate with ampicillin and Xgal in each of these scenarios:a) transformation not successful (meaning the plasmid did not go inside the bacteria)b) transformation was successful, but no insert in LacZ gene (plasmid went in the bacteria but there was no inserted DNA in the LacZ gene of the plasmid)c) transformation and the ligation reactions were successful (got the plasmid in, and the plasmid is carrying extra DNA in the LacZ region, yay)arrow_forwardReflect on this "Gene therapy is still in its infancy, but its believe that as it matures, it will become an effective treatment for the myriad of genetic diseases that effect humanity ?arrow_forward
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