Question asked to listextra sequences that are necessary in a YAC vector so that it can actsimilar to an artificial chromosome and question also asked to describe the significance of each essential sequence. Introduction: Genetically designed chromosomes obtained from DNA of yeast are termed as Yeast Artificial Chromosomes (YAC). Many difficulties related to classical chromosomal walking, in which the conventional plasmids were used, can be overcome by using YAC, because it can clone large sizeof DNA 100-1000kb . Necessary characters of cloning vectors are- it has ori site (site of replication), selectable marker, and possesses a multiple cloning site. Conventional plasmids are the vectors used for cloning the DNA piece and also termed as traditional plasmids.

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Chapter 22, Problem 15EQ

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Question asked to listextra sequences that are necessary in a YAC vector so that it can actsimilar to an artificial chromosome and question also asked to describe the significance of each essential sequence.

Introduction:

Genetically designed chromosomes obtained from DNA of yeast are termed as Yeast Artificial Chromosomes (YAC). Many difficulties related to classical chromosomal walking, in which the conventional plasmids were used, can be overcome by using YAC, because it can clone large sizeof DNA 100-1000kb.

Necessary characters of cloning vectors are- it has ori site (site of replication), selectable marker, and possesses a multiple cloning site. Conventional plasmids are the vectors used for cloning the DNA piece and also termed as traditional plasmids.

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