Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 22, Problem 19EQ
A human gene, which we will call gene X, is located on chromosome 11 and is found as a normal allele and a recessive disease-causing allele. The location of gene X has been approximated on the map shown here, which contains four STSs, labeled STS-1, STS-2, STS-3, and STS-4.
A. Explain the general strategy of positional cloning.
B. If you applied the approach of positional cloning to clone gene X, where would you begin? As you progressed in your cloning efforts, how would you know if you were walking toward or away from gene X?
C. How would you know if you had reached gene X? (Keep in mind that gene X exists as a normal allele and a disease-causing allele.)
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Students have asked these similar questions
a. What is the purpose of molecular cloning?b. What purpose do selectable markers serve in vectors?c. What is the purpose of the origin of replication in aplasmid vector?d. Why do cloning vectors have polylinkers?
5) Below is an image that shows both reproductive and therapeutic cloning. Use this image to answer compare and contrast therapeutic and reproductive cloning. Are they used for similar means…etc. Once you have done that answer the question below.
a) There are two types of therapeutic cloning. What are they and how are they different?
Dolly is the first mammal to have been successfully cloned from an adult cell Which of the following statement/s is/are most relevant to the birth of Dolly?
I. It suggests that human could be cloned.
II. It proves that specialized cells could be used to create an exact copy of the animal they came from.
III. It improves the production of milk, meat, and other products from livestock.
IV. It proves that animals could be cloned to have gene mutations that help scientists study diseases that develop in the animals.
A. II only
B. I and II
C. III, and IV
D. II, III, and IV
Which of the following statements best explain the significance of mitosis and ?
A. Both mitosis and meiosis produce diploid cells which responsible for the continuity of life.
B. Many single-celled organisms rely on mitosis and meiosis as their primary means of asexual reproduction
C. replication, cells have another interesting choice, whether they want to make an identical copy, or do they want to make four half-copies…
Chapter 22 Solutions
Genetics: Analysis and Principles
Ch. 22.1 - Prob. 1COMQCh. 22.2 - Prob. 1COMQCh. 22.3 - A molecular marker is a _____ found at a specific...Ch. 22.3 - 2. Which of the following is an example of a...Ch. 22.3 - To map the distance between molecular markers via...Ch. 22.4 - 1. What is a contig?
a. A fragment of DNA that...Ch. 22.4 - A vector that can carry a large fragment of...Ch. 22.4 - 3. Chromosomal walking is a method of _____ in...Ch. 22.5 - Prob. 1COMQCh. 22.5 - Prob. 2COMQ
Ch. 22.5 - 3. A prokaryotic genome is about 4 million bp in...Ch. 22.6 - Metagenomics is aimed at a. determining the...Ch. 22 - 1. A person with a rare genetic disease has a...Ch. 22 - For each of the following, decide if it could be...Ch. 22 - Which of the following statements about molecular...Ch. 22 - 1. Is each of the following a method used in...Ch. 22 - Prob. 2EQCh. 22 - Prob. 3EQCh. 22 - The cells from a persons malignant tumor were...Ch. 22 - 5. Figure 23.2 describes the technique of FISH....Ch. 22 - Explain how DNA probes with different fluorescence...Ch. 22 - 7. A researcher is interested in a gene found on...Ch. 22 - Prob. 8EQCh. 22 - Prob. 9EQCh. 22 - Prob. 10EQCh. 22 - Prob. 11EQCh. 22 - Prob. 12EQCh. 22 - In the Human Genome Project, researchers have...Ch. 22 - 14. Take a look at question 3 in More Genetic...Ch. 22 - 15. Place the following stages of a physical...Ch. 22 - 16. What is an STS? How are STSs generated...Ch. 22 - 17. Four cosmid clones, which we will call cosmids...Ch. 22 - A human gene, which we will call geneX, is located...Ch. 22 - 19. Describe how you would clone a gene by...Ch. 22 - 20. A bacterium has a genome size of 4.4 Mb. If a...Ch. 22 - 21. Discuss the advantages of next-generation...Ch. 22 - Prob. 23EQCh. 22 - Prob. 24EQCh. 22 - Prob. 15EQCh. 22 - What is a molecular marker? Give two examples....Ch. 22 - Which goals of the Human Genome Project do you...
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- List the three features of a cloning vector and why these elements are neededarrow_forwardUsing illustrations describe the structure of a typical cloning vector and discuss the functions of each element.arrow_forwardName any two cloning vectors. Describe the features required to facilitate cloning into a vector.arrow_forward
- Describe three possible uses of site-directed mutagenesis.arrow_forwardThe goal of most gene therapies is to insert a healthy copy of a gene into the genome. Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location and thus different cells end up with the DNA sequence in different positions. A. It has been shown that where the healthy gene integrates is heavily influenced by chromatin. Why? B. The same gene integrated into different locations in the genome will be expressed differently. Why? C. Over time the expression of an integrated gene will change, usually decreasing. Describe how you think the cell downregulates expression of an integrated gene.arrow_forwardA graduate student isolates two new retroviruses: Virus A and Virus B. To determine whether these viruses could cause cancer, the student infected human cells with each of the viruses and then transfered the infected cells to nude mice to see if the viruses would cause formation of tumors. The student was able to determine by PCR that both viruses are integrated into the genome of the host cells before transplanting cell in mice. The data for this assay are shown in the figure below. Explain how the mechanism of transduction may be different for these two viruses to yield the observed data (limit 4-5 sentences). Retrovirus Transformation Assay % Cells Cause Tumors 120 100 80 60 40 20 0 0 10 -Virus A -Virus B 30 20 Days Post Infection 40arrow_forward
- You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a. Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above,arrow_forwardOne concern about using genetically-modified organisms is that many of the methods used to create them introduce into the genome DNA from a different species (i.e. foreign DNA). Which of the following methods has the lowest potential of introducing foreign DNA into the genome?A. A gene knockout in mouse using homologous recombination in ES cellsB. Introduction of a P element vector into the Drosophila germ-lineC. Deletion mutations introduced by CRISPR/Cas9D. Microinjection of a transgene into a mouse pronucleusarrow_forwardMatch items in Column A with most related ones in B. Write the letters on the space provided.arrow_forward
- a) Restriction enzymes are essential tools in genetic engineering. Discuss with diagrams b)Define the term genetic engineering and in a tabular form outline the differences between genetic recombination and genetic engineeringarrow_forwardA has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forwardDescribe the important features of cloning vectors. Explain the purpose of selectable markers in cloning experiments.arrow_forward
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