Concept explainers
(a)
To describe: The reason why
Introduction:
Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.
(b)
To determine: In gel B, whether the DNA from the
Introduction:
Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.
(c)
To determine: The reason for the presence of relaxed DNA in these lanes. As given, in both gels, the
Introduction:
Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.
(d)
To determine: The approximate superhelical density of the native DNA.
Introduction:
Gel electrophoresis is a technique by which the DNA fragments could be separated based on their size and charge. The samples are loaded in the well which is separated based on their charge in between the positive and negative electrodes. When the current passes through the gel, molecules moves across the gel according to their charge.
Want to see the full answer?
Check out a sample textbook solutionChapter 24 Solutions
Lehninger Principles of Biochemistry
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON