Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 24, Problem 9P
Summary Introduction

To determine: The chromatin structure defined by the given gel picture.

Introduction:

The term chromosome defines a nucleic acid molecule which transfers the genetic information in an archeon, a virus, a bacterium, and a eukaryotic cell. The structure of chromatin consists of DNA molecules along with proteins and small amount of RNA. DNA present in the chromatin is tightly associated with histone proteins. Histone protein causes the packaging of DNA into structural units, termed as nucleosomes. Other proteins present in chromatin maintain the structure of chromosome and regulation of genes. Nucleosomes are the basic units of chromatin structures.

Summary Introduction

To determine: The reason why the DNA bands are thick and spread out rather than sharply defined.

Introduction:

Agarose gel electrophoresis is an essential method for separating fragments of DNA of varying sizes that ranges between 100 bp to 25 kb. Movement of DNA fragments in the agarose gel depends on their mass and size ratio.

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