BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
9th Edition
ISBN: 9781319425784
Author: BERG
Publisher: Macmillan Higher Education
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Chapter 26, Problem 35P
Interpretation Introduction
Interpretation:
The explanation for the inhibition of enzyme activity by the phosphorylation of the serine residue should be determined.
Concept introduction:
The steroid can be defined as a
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disease. As such, a frontline treatment for Type 2 diabetes is the drug metformin, which acts indirectly
to inhibit gluconeogenesis in the liver. You are a research biochemist who would like to develop new
drugs that act to directly inhibit gluconeogenesis. You have just gained access to a library of thousands
of small molecules of unknown activity, and you would like to identify lead compounds that have specific
inhibitory activity against steps in the gluconeogenesis pathway.
(a)
into PEP in order to screen for inhibitors of enzymes specific to gluconeogenesis. Which enzymes do
you need to purify, what cofactors and allosteric effectors do they require, and which reactants do you
need to add to reconstitute the reactions for the first bypass? Which intermediates and products are
generated?
Your first approach is to reconstitute the initial set of bypass reactions that convert pyruvate
(b)
vitro reconstitution?
What additional steps and enzymes are required in liver cells but are…
. Pyruvate can be processed under anaerobic conditions to ethanol (in yeast) or to lactate (in
mammals), as shown.
Explain the primary purpose of these reactions.
Describe the major biochemical features of each reaction
Closely related. Pyruvate dehydrogenase complex and a-ketoglutarate
a-ketoglutarate dehydrogenase complex are huge enzymes
consisting of three discrete enzymatic activities. Which amino acids
require a related enzyme complex, and what is the name of the
enzyme?
Chapter 26 Solutions
BIOCHEMISTRY (LOOSELEAF)-W/ACCESS
Ch. 26 - Prob. 1PCh. 26 - Prob. 2PCh. 26 - Prob. 3PCh. 26 - Prob. 4PCh. 26 - Prob. 5PCh. 26 - Prob. 6PCh. 26 - Prob. 7PCh. 26 - Prob. 8PCh. 26 - Prob. 9PCh. 26 - Prob. 10P
Ch. 26 - Prob. 11PCh. 26 - Prob. 12PCh. 26 - Prob. 13PCh. 26 - Prob. 14PCh. 26 - Prob. 15PCh. 26 - Prob. 16PCh. 26 - Prob. 17PCh. 26 - Prob. 18PCh. 26 - Prob. 19PCh. 26 - Prob. 20PCh. 26 - Prob. 21PCh. 26 - Prob. 22PCh. 26 - Prob. 23PCh. 26 - Prob. 24PCh. 26 - Prob. 25PCh. 26 - Prob. 26PCh. 26 - Prob. 27PCh. 26 - Prob. 28PCh. 26 - Prob. 29PCh. 26 - Prob. 30PCh. 26 - Prob. 31PCh. 26 - Prob. 32PCh. 26 - Prob. 33PCh. 26 - Prob. 34PCh. 26 - Prob. 35PCh. 26 - Prob. 36PCh. 26 - Prob. 37PCh. 26 - Prob. 38PCh. 26 - Prob. 39PCh. 26 - Prob. 40PCh. 26 - Prob. 41PCh. 26 - Prob. 42PCh. 26 - Prob. 43PCh. 26 - Prob. 44PCh. 26 - Prob. 45PCh. 26 - Prob. 46PCh. 26 - Prob. 47P
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