Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 26, Problem 4AL

You are studying RNA viruses and have discovered a new one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don’t know if it is plus or minus stranded. Your lab-mate says, “Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome.” You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate’s suggestion?

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You are studying RNA viruses and have discovered a new one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don’t know if it is plus or minus stranded. Your lab-mate says, “Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome.” You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate’s suggestion?
The smallpox virus, which actually has a fairly large genome size for a virus (about 190,000 base pairs), must use which of the following enzymes to replicate its nucleic acid? A. DNA-dependent-DNA-polymerase B. RNA-dependent-DNA-polymerase C. DNA-dependent-RNA-polymerase D. RNA-dependent-RNA-polymerase E. reverse transcriptase
Which of the following experimental results was NOT evidence that DNA is the genetic molecule rather than proteins?   a. When a virus was radioactively labelled, and the virus was allowed to infect a bacteria cell, radioactive virus DNA was found inside the bacteria cell while radioactive virus proteins were found outside the cell.   b. Dead pathogenic bacteria cells combined with living nonpathogenic bacteria cells caused the creation of living pathogenic cells and thus the death of the host animal.   c. Proteins are a class of macromolecules with the diversity and specificity needed for hereditary material.   d. Nucleotide bases occur regularly, such that the number of adenines = number of thymines, and the number of guanines = number of cytosines.

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Prescott's Microbiology

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