Campbell Essential Biology with Physiology (5th Edition)
Campbell Essential Biology with Physiology (5th Edition)
5th Edition
ISBN: 9780321967671
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece, Kelly A. Hogan
Publisher: PEARSON
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Chapter 3, Problem 16PS
Summary Introduction

To describe: Tests that can help in determining which versions of lactose having an altered amino acid residue have a significantly altered three-dimensional structure.

Introduction:

Lactase in a digestive enzyme found in the human body. It hydrolyzes a disaccharide called lactose. Lactose is a disaccharide found richly in milk. Its hydrolysis yields two monosaccharides called glucose and galactose. Basically, the proteins are three-dimensional structures that are denoted by the order of the amino acids in its chain. The final conformational folded structure is driven by any polypeptide chain.

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Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids. ___1___--_2_- 3 -- --____6_--_7__ a. The amino acid in the C-terminal is optically inactive. b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein but produces a yellow soln with basic hydrolysate. It also gives a negative result with both Hopkin's Cole and Millon's test using basic hydrolysate. c. After treatment of this peptide with aminopeptidase (specificity: peptide bond on the N-terminus), it yields serine and a hexapeptide d. The amino acid besides the N-terminal amino acid. will yield ornithine and urea after alkaline hydrolysis. e. The third amino acid gives a positive result for Hopkins-Cole Test. f. Chymotrypsin^ cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue. g. The amino acid at the carboxy-end of the third amino acid will yield a…
Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids. a. The amino acid in the C-terminal is optically inactive. b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein while producing a yellow soln with Xanthoproteic reagent and yielding a negative result with Hopkin's Cole test using basic hydrolysate. c. Yields serine and a hexapeptide after treatment of aminopeptidase (specificity: peptide bond on the N-terminus). d. The amino acid beside the N-terminal a.a. will yield ornithine and urea after alkaline hydrolysis. e. The third amino acid gives a positive result for Hopkins-Cole Test. f. Chymotrypsin cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue. g. The amino acid at the carboxy-end of the third a.a. will yield a black precipitate for Pb(Ac)2.
A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol, three bands are again formed, this time with molecular masses of 160, 90, and 60 kDa. Determine the subunit composition of the protein and clearly explain how you came to this determination.
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