Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 3, Problem 19E
Summary Introduction

To determine: The restriction enzymes would interfere with the production of β-galactosidase by the host cell.

Concept introduction: The plasmid pUC18 is a circular plasmid that contains multiple restriction sites. In pUC18, the enzyme β-galactosidase is encoded by the lacZ gene. The β-galactosidase cleaves the colorless compound X-gal to a blue-colored product.

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Chapter 3 Solutions

Fundamentals of Biochemistry: Life at the Molecular Level

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