Concept explainers
(a)
To describe: The possible products of a PCR in which one of the primers is advertently lost from the reaction mixture.
Concept introduction:
The
(b)
To describe: The possible products of a PCR experiment in which one of the primers is complementary to several sites in the starting DNA sample.
Concept introduction:
The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). The primers incorporated with the DNA polymerase to synthesize complementary strands of the target DNA.
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