Chapter 3, Problem 36CQ

(a)

Summary Introduction

To describe:  The possible products of a PCR experiment which carry  a single stranded disruption in the target DNA sequence, which is present in only one copy in the starting sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). A PCR cycle consists of three steps such as strand separation, the hybridization of primers, and the extension of by DNA synthesis.

(b)

Summary Introduction

To describe:  The possible products of a PCR experiment which carry  a double stranded break in the target DNA sequence, which is present in only one copy in the starting sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotides triphosphate (dNTPs). A PCR cycle consists of three steps such as strand separation, the hybridization of primers, and the extension of by DNA synthesis.