Chapter 3, Problem 38CQ

(a)

Summary Introduction

To explain: The reason for the presence of only one allele at some loci.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated by using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphates (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR amplified products are subjected to electrophoretograms to identify the alleles.

(b)

Summary Introduction

To identify: The suspect that could be a possible source of the blood sample.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphates (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.

(c)

Summary Introduction

To determine: Whether the suspect could be recognized using one of the three given STR loci.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphate (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.

(d)

Summary Introduction

To compare: The amount of DNA obtained from suspect 1 with suspect 4.

Concept introduction:

The polymeric chain reaction (PCR) is a technique in which amplification of a specific DNA segment occurs quickly and accurately. In this technique, a DNA sample is separated using restriction enzymes into single strands. Then, these strands are incubated with DNA polymerase such as Taq polymerase, two primers, and deoxynucleotide triphosphate (dNTPs). Different types of fluorescent primers are used in PCR, and the PCR-amplified products are subjected to electrophoretograms to identify the alleles.