Chapter 3, Problem 5P

(a)

Summary Introduction

To determine: The amino acid in-between aspartate and lysine which would be eluted first from cation-exchange column by a $\text{pH}$7.0 buffer.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Cation-exchange chromatography and anion-exchange chromatography are the two types of ion-exchange chromatography. Cation-exchange chromatography is used to separate positively-charged molecules.

(b)

Summary Introduction

To determine: The amino acid in-between arginine and methionine which would be eluted first from cation-exchange column by a $\text{pH}$ 7.0 buffer.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Cation-exchange chromatography and anion-exchange chromatography are the two types of ion-exchange chromatography. Cation-exchange chromatography is used when the molecule to be separated is positively charged.

(c)

Summary Introduction

To determine: The amino acid in-between glutamate and valine which would be eluted first from cation-exchange column by a $\text{pH}$ 7.0 buffer.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Cation-exchange chromatography and anion-exchange chromatography are the two types of ion-exchange chromatography. Cation-exchange chromatography is used when the molecule to be separated is positively charged.

(d)

Summary Introduction

To determine: The amino acid in-between glycine and leucine which would be eluted first from cation-exchange column by a $\text{pH}$ 7.0 buffer.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be separated with the help of ion exchange chromatography. Cation-exchange chromatography and anion-exchange chromatography are the two types of ion-exchange chromatography. Cation-exchange chromatography is used when the molecule to be separated is positively charged.

(e)

Summary Introduction

To determine: The amino acid in-between serine and alanine what would be eluted first from cation-exchange column by a $\text{pH}$ 7.0 buffer.

Introduction:

In ion-exchange chromatography, polar molecules or ions are separated on the basis of their affinity to ion exchanger. Proteins, amino acids, and small nucleotides can be seprated with the help of ion exchange chromatography. Cation-exchange chromatography and anion-exchange chromatography are the two types of ion-exchange chromatography. Cation-exchange chromatography is used when the molecule to be separated is positively charged.