Concept explainers
(a)
Interpretation:
The autoradiographic pattern for strands (i) to (v) should be determined.
Concept introduction:
DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.
RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The
(b)
Interpretation:
The possible effect of rifampicin on the RNA synthesis in the given system should be determined.
Concept introduction:
DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.
RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.
(c)
Interpretation:
The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.
Concept introduction:
DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.
RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.
(d)
Interpretation:
The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.
Concept introduction:
DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.
RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.
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BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
- please help me with this question. As this is a non-directional cloning, recombinant plasmids can contain an insert ligated into the vector in two different orientations. Provide two diagrams to illustrate the two potential recombinant plasmids, with the inserts ligated in opposite orientations. Include all RE sites and distances between sites on the diagram.arrow_forwardPlease help me solve this problem. I am really having a hard time understanding this lesson. Please help. Kindly provide all the necessary information to this problem. Thank you! Please answer numbers 1-5 determine what amino acid will be formed from the given DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ Note: Prepare the partner strand of this DNA. Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Partner DNA strand 2. the mRNA strand 3. The tRNA 4. the formed amino acids 5. the discussion of the entire procedurearrow_forwardRNA is transcribed. Label the 5′ and 3′ ends of each strand. 17. The following sequence of nucleotides is found in a single-stranded DNA template: ATTGCCAGATCATCCCAATAGAT Assume that RNA polymerase proceeds along this template from left to right. a. Which end of the DNA template is 5′ and which end is 3′? b. Give the sequence and identify the 5′ and 3′ ends of the RNA transcribed from this template.arrow_forward
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning