BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 32, Problem 8P
Interpretation Introduction
Interpretation:
Differences between the -10 and -35 sequences of λ repressor and Cro genes should be determined.
Concept introduction:
λ repressor inactivated state binds to OR1 and OR2 sites and inhibits the transcription of phage gene. On the other hand, Cro protein binds to the same sites, to which λ repressor binds. In this way, the Cro protein inhibits the action of λ repressor.
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You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase).(a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities.
Broken operators. Consider a hypothetical mutation in OR2OR 2 that
blocks both A repressor and Cro binding. How would this mutation
affect the likelihood of bacteriophage entering the lytic phase?
Yes or no only.
rna seq can provide sequence and expression data
do riboprobes synthesize bu in vitro transcription?
does rna causes mutations and lose of function of specific genes?
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