BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
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Chapter 35, Problem 11P
Interpretation Introduction
Interpretation:
The reason for the lack of response to gram-negative bacteria due to a mutation in TLR4 should be determined.
Concept introduction:
The TLRs are the toll-like receptors thatplay an important role in the innate responses of the immune system. They are membrane-spanning, non-catalytic receptors. LPS is lipopolysaccharide that forms the outer membrane of the gram-negative bacteria.
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It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
In own word. What are the differences between contagious and communicable diseases? Explain with example?
Chapter 35 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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