BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
9th Edition
ISBN: 9781319425746
Author: BERG
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 35, Problem 10P
Interpretation Introduction
Interpretation:
The purpose of the hybridization of T-cell cDNA with B-Cell mRNA should be determined.
Concept introduction:
The cloning is the technique of production of the gene of desire in another cell. In this, the entire genome or a segment of the genome can be inserted in other cells, where it expresses the desired trait.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
My hypothesis:
The Hyper-IgE syndrome, also known as Job’s syndrome, is an immunodeficiency disease resulting from the lack of function of a single gene (gene ‘X’). This Hyper IgE/Job's syndrome or STAT3 deficiency(HIES) is caused by STAT3 gene mutation. STAT3 is a gene involved in major signal transduction pathways including wound healing, angiogenesis, immune response, and allergies. This genetic mutation is autosomal dominant in nature. The above mentioned are the most likely immune function impaired in the Gene X-deficient patients including immunity with eczematous and non-immunologic system disorders.
Question:
In a lab, histological examination of tongue sections from Candida albicans infected mice were examined, and the numbers of infiltrating leukocytes (white blood cells) were quantified in each microscopic field of each section, and the results are shown in the figure below. Do these data support or refute my hypothesis stated above? Why or why not?
Gene Expression and the Impact of a Mutation.
Can someone help me to answer the question 8 and 9, please?
8. How has the mutation altered the polypeptide? Is the function of the hemoglobin molecule (which includes 2 ẞ-globin polypeptides and 2 a-globin polypeptides) impaired? (Read your book to learn more about sickle cell disease.)
9. What is the relationship between the genotype in this case and the individual's phenotype?
asap please
Can you please help me by drawing a serie of schematic figures that demonstrates the information in the paragraph below.
The carboxy terminus of the p53 protein acts as an allosteric regulator of sequence-specific DNA binding. This was demonstrated initially by Hupp et al. (1992) using a bacterially expressed protein. Recombinant bacterial p53 bound poorly to DNA, and binding could be enhanced by the addition of antibodies specific to the C-terminal region of the protein. Phosphorylation of Ser315 and Ser392 within this domain also enhance sequence-specific DNA binding. Dephosphorylation of Ser376 of p53 after IR allows the association of 14-3-3 proteins with the C terminus of the protein (Waterman et al., 1998). Stavridi et al. (2001) demonstrate that this interaction is required for p53 to activate the downstream gene, p21waf1/cip1, and for the G1 cell cycle checkpoint arrest response. Interestingly, this dephosphorylation event seems to be ATM-dependent, possibly by a phosphatase…
Chapter 35 Solutions
BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Matching Match each item with the correct statement below. Not all terms will be used a. 5' GTP cap f. RFLPs b. Target copy g. sticky ends c. Ligase h. cloned DNA d. Cas-9 i recombinant DNA j. ampicillin e. Host cell 1. A cell (usually a bacteria) that has a gene put in it so the gene can be cloned 2. An example of a post-transcriptional modification of mRNA 3. Sections of DNA that vary between individuals. These can be used for DNA fingerprinting 4. May be used following transformation to kill off cells that a gene didn't enter 5. Molecule of DNA created during PCR that is the desired length 6. A molecule of DNA that comes from two different sources that are spliced together 7. Ends of DNA cut by a restriction enzyme where not all nucleotides are paired (i.e. ends are uneven) 8. Protein that works with CRISPR to cut DNA at a specific spotarrow_forwarddraw the p21 promoter. Your drawing should include (1) the start site, (2) the TATA box and (3) the ERE/AP-1 binding sitearrow_forwardtransformation and CRISPR. In your own words, briefly describe two differences between these technologies. (For example, these can be differences between their outcomes, procedures, reagents, or something else.)arrow_forward
- True or False? Eukaryotic genomes are organized into operons; each operon consists of a series of genes which code for enzymes involved in a metabolic pathway, under the transcriptional control of a single promoter sequence .arrow_forwardThe trp operon vs. the lac operon: Which one of these is activated in the presence of a substance, and which is activated in the absence, and why the difference? In other words, what if the cell could think, what would be its rationale for why one is activated in the presence and the other in the absence of something? (Ignore the effects of glucose in the lac operon.)arrow_forwardHuman cells are highly resistant to transformation. Experiments have shown that 5 regulatory circuits (pathways) have to be altered before human cells can grow as tumor cells in immunocompromised mice. State each of these circuits. Explain how the alteration of the protein of that particular circuit leads to uncontrolled growth.arrow_forward
- Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator +1 lac Y lac A DNA 1. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 2. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 3. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 4. Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization proteins when lactose is not available or when glucose is present? 5. Why is it adaptive for the structural genes for using lactose to be under the control of a single promoter, i.e., synthesize a polycistronic message rather than three monocistronic message?arrow_forwardcan you please help me out with this ? Mutations in the IL2RG gene cause approximately 30 percent of severe combined immunodeficiency disorder (SCID) cases in humans. These mutations result in alterations to a protein component of cytokine receptors that are essential for proper development of the immune system. The IL2RG gene is composed of eight exons and contains upstream and downstream sequences that are necessary for proper transcription and translation. Below are some of the mutations observed. For each, explain its likely influence on the IL2RG gene product (assume its length to be 375 amino acids). Nonsense mutation in a coding region Insertion in Exon 1, causing frameshift Insertion in Exon 7, causing frameshift Missense mutation Deletion in Exon 2, causing frameshift Deletion in Exon 2, in frame (g) Large deletion covering Exons 2 and 3arrow_forwardExpression and Silencing of GFP 1. Would the oligonucleotide primers used in Lab 3 to detect the GFP transgene and GFP MRNA also detect the GFP hairpin transgene in pUQC218?arrow_forward
- Yes or no? reverse genetics is RNA interference example. cellular differentiation potency in multipotent is greater than pluripotent stem cell. does digoxigenin UTO use to make dsrna and perform rna interference?arrow_forwardRegulation of Genes and Their products 1. Given the following genotypes, explain how the mutation (identified by a (-) superscript) wil affect E. coll grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the lac operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not? a. I+p+o+z- y+ b. i- p+o+z+y+ c. i+p+o- z+y+ d. i+p- o+z+y+ 2. In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan. 3. Experiments show that mutations at gene E lead to non-repressible transcription of trp genes. Why?arrow_forwardPlease help me with this question. More than one answer may be correct. A greater number of protocadherin genes ____. Options: A) are found in Drosophila than humans B) were the precursors to megacadherin, which eventually defeated Dr. Wily C) are present in vertebrates than classical cadherin genes D) are associated with larger brains E) are found in octopuses than humansarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningHuman Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning