Concept explainers
(a)
Interpretation:
The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.
Concept introduction:
Nucleotides are the construction buildings of
(b)
Interpretation:
A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.
Concept introduction:
DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the
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BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
- typical lab bacterial species that grows at 37°C. Explain your reasoning. 13. One strand of DNA reads as follows: 5'-ATG GCG CGA CTG ACT-3' A. How many nucleotides are listed here? B. What are the three structural components of one nucleotide? C. Write the appropriate sequence for the complementary strand above or below the sequence shown. Be sure to include which end of the complementary strand is 5' and which end is 3'.arrow_forwarddo answer correctly and explain.arrow_forwardPstl. EcoRI Origin of replication (ori) Ampicillin Tetracycline resistance resistance (Amp) (Tet") pBR322 (4,361 bp) BamHI Pvull Sall Recombinant Plasmid DNA Bacterial cell contains... No plasmid DNA pBR322 (no insert) Recombinant plasmid 00,000. Host DNA Transformation of E. coli cells +AMP plate pBR322 Figure 7-5 +TET plate Based on the recombinant plasmid growth pattern (bottom row of blue table), which of the depicted plasmid's restriction sites was used to prepare this sample? Explain how you can tell.arrow_forward
- match.arrow_forwardPlease ASAP. Thank youarrow_forwardP2. Calculate the frictional coefficient of a molecule of DNA of 20 base pairs in water at 20C; assume that the hydrodynamic behavior of the DNA itself can be approximated to be rod-like; the viscosity of the solvent is 0.01 g cm-1 s -1arrow_forward
- . Explain why DNA is stable in the presence of alkali (0.3 M KOH), while RNA is quantitatively degraded to 2'- and 3'-nucleoside monophosphates under these conditions.arrow_forwardB PLEASE second onearrow_forwardYou have another circular plasmid. Complete and effective digestion of this plasmid with a restriction enzyme yields three bands: 4kb, 2kb, and 1 kb. In comparing the band intensity on an ethidium bromide-stained gel, you notice that the 4 kb and the 2 kb bands have the exact same brightness. The 1 kb band is exactly one fourth as bright as each of these. (Assume there is uniform staining with ethidium bromide throughout the gel.) How many times did the enzyme cut the plasmid? What is the size of the plasmid? Justify your answers to a and b above using a clearly labeled diagram showing the relative location of the cut-sites on the plasmid.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning