BIOCHEMISTRY BOOKS ALC&MOD MST/ET PKG
1st Edition
ISBN: 9780134172507
Author: APPLING
Publisher: Pearson Education
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Textbook Question
Chapter 5, Problem 12P
a. Write a possible sequence for an mRNA segment coding for apamine.
b. Do you think apamine is synthesized in the form shown in Problem 11, or is it more likely a product of proteolytic cleavage of a larger peptide? Explain.
11. Apamine is a small protein toxin present in the venom of the honeybee. It has the sequence
CNCKAPETALCARRCQQH
a. If apamine does not react with iodoacetate (see Tools of Biochemistry 5B), then how many disulfide bonds are present?
b. If trypsin cleavage gave two peptides, then where is(are) the S-S bond(s)?
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Check out a sample textbook solutionStudents have asked these similar questions
. Why is a nonsense suppressor tRNATyr, even though ithas a mutant anticodon that cannot recognize a tyrosinecodon, charged with tyrosine by Tyr tRNA synthetase?
A. What amino acid sequence is encoded by the codon sequence AUAAUGGUAACGGUU?
B. Suppose the codon sequence AGACACUCUAUUAAA
has a single base pair mutation to AGACACUCUUUUAAA.
If the old protein sequence was Arg-His-Ser-Ile-Lys, what will be the new sequence encoded by the mutant gene?
A peptide with the primary structure Lys-Arg-Pro-Leu-Ile-Asp-Gly-Ala must be synthesized by the methods developed
by Merrifield.
Calculate the percentage of the peptides synthesized that will
be full length and have the correct sequence if the addition of
each amino acid residue is 96% efficient.
Do the calculation a second time but assume a 99% efficiency
for each cycle.
full-length peptides with the
correct sequence if 96% efficient:
full-length peptides with the
correct sequence if 99% efficient:
%
%
Chapter 5 Solutions
BIOCHEMISTRY BOOKS ALC&MOD MST/ET PKG
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